SIRT6 Flag vector (V007170)

MSDS

Basic Vector Information

- - Vector Name:
  - SIRT6 Flag
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 6486 bp
- - Type:
  - Mammalian Expression
- - Replication origin:
  - ori
- - Selection Marker:
  - Neomycin (select with G418)
- - Copy Number:
  - High Copy
- - Promoter:
  - CMV
- - Cloning Method:
  - Restriction Enzyme
- - 5' Primer:
  - T7
- - 3' Primer:
  - BGH Reverse

SIRT6 Flag vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

SIRT6 Flag vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       SIRT6_Flag.        6486 bp DNA     circular SYN 20-DEC-2021
DEFINITION  Mammalian expression of human SIRT6 with flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    SIRT6 Flag.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   North BJ, Marshall BL, Borra MT, Denu JM, Verdin E
  TITLE     The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin 
            deacetylase.
  JOURNAL   Mol Cell. 2003 Feb . 11(2):437-44.
  PUBMED    12620231
REFERENCE   2  (bases 1 to 6486)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6486)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2003 Feb . 11(2):437-44."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(9..32)
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(45..1109)
                     /gene="SIRT6"
                     /label=SIRT6
                     /note="NAD-dependent protein deacylase sirtuin-6 from Homo 
                     sapiens. Accession#: Q8N6T7"
     promoter        complement(1170..1188)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1233..1436)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1437..1816)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1988..2007
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     promoter        2082..2186
                     /label=AmpR promoter
     CDS             2187..3044
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3218..3806
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3960..3977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4094..4115
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4130..4160
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4168..4184
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4192..4208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(4245..4378)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4555..5346)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5413..5742)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(5756..6184)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(6230..6454)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"

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