MSGV1-1D3-28Z.1-3 mut vector (V012151)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012151 MSGV1-1D3-28Z.1-3 mut In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

MSGV1-1D3-28Z.1-3 mut contains an anti-mouse CD19 CAR with CD3 ITAMs mutated.

      • Vector Name:
      • MSGV1-1D3-28Z.1-3 mut
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6941 bp
      • Type:
      • Retroviral
      • Cloning Method:
      • Restriction Enzyme
      • Growth Strain(s):
      • stbl3
      • Growth Temperature:
      • 37℃

MSGV1-1D3-28Z.1-3 mut vector Vector Map

MSGV1-1D3-28Z.1-3 mut6941 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069005' LTRpLXSN 5'pBABE 5'pBMN 5'Kozak sequence3' LTRlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterpBRforEcopGEX 3'pRS-markerIn lacZ genepBRrevBam

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells. Blood. 2010 Nov 11;116(19):3875-86.

MSGV1-1D3-28Z.1-3 mut vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6941 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Anti-mouse CD19 CAR with CD3 ITAMs mutated.
ACCESSION   .
VERSION     .
KEYWORDS    MSGV1-1D3-28Z.1-3 mut
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA
  TITLE     Adoptive transfer of syngeneic T cells transduced with a chimeric 
            antigen receptor that recognizes murine CD19 can eradicate lymphoma 
            and normal B cells.
  JOURNAL   Blood. 2010 Nov 11;116(19):3875-86. doi: 
            10.1182/blood-2010-01-265041. Epub 2010 Jul 14.
  PUBMED    20631379
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2010-01-265041"; journalName: "Blood"; date: 
            "2010-11-11- 11"; volume: "116"; issue: "19"; pages: "3875-86"
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     1357..1379
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1395..1411
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1749..1765
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1822..1831
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     LTR             3326..3840
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    4012..4028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4036..4066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4081..4102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4219..4236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4390..4978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4470..4489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5149..6009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5772..5791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6010..6114)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6182..6200
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6238..6260)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6360..6379
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6573..6595
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6723..6742
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"