MSGV1-1D3-28Z.1-3 mut vector (V012151)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012151 MSGV1-1D3-28Z.1-3 mut In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

MSGV1-1D3-28Z.1-3 mut contains an anti-mouse CD19 CAR with CD3 ITAMs mutated.

      • Vector Name:
      • MSGV1-1D3-28Z.1-3 mut
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6941 bp
      • Type:
      • Retroviral
      • Cloning Method:
      • Restriction Enzyme
      • Growth Strain(s):
      • stbl3
      • Growth Temperature:
      • 37℃

MSGV1-1D3-28Z.1-3 mut vector Vector Map

MSGV1-1D3-28Z.1-3 mut6941 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069005' LTRgag (truncated)pol regionKozak sequence3' LTRlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterpBRforEcopGEX 3'pRS-markerIn lacZ genepBRrevBam

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells. Blood. 2010 Nov 11;116(19):3875-86.

MSGV1-1D3-28Z.1-3 mut vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2079        6941 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Anti-mouse CD19 CAR with CD3 ITAMs mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA
  TITLE     Adoptive transfer of syngeneic T cells transduced with a chimeric 
            antigen receptor that recognizes murine CD19 can eradicate lymphoma 
            and normal B cells.
  JOURNAL   Blood. 2010 Nov 11;116(19):3875-86. doi: 
            10.1182/blood-2010-01-265041. Epub 2010 Jul 14.
  PUBMED    20631379
REFERENCE   2  (bases 1 to 6941)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6941)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2010-01-265041"; journalName: "Blood"; date: 
            "2010-11-11- 11"; volume: "116"; issue: "19"; pages: "3875-86"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     CDS             1020..1427
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="VTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTFNVG
                     WPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPP
                     LPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1437..1807
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     regulatory      1822..1831
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             3326..3840
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(4012..4028)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4036..4066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4081..4102)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(4219..4236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4390..4978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5152..6009)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6010..6114)
                     /label=AmpR promoter
     primer_bind     6182..6200
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     primer_bind     complement(6238..6260)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6360..6379
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     6573..6595
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6723..6742
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"