Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V012151 | MSGV1-1D3-28Z.1-3 mut | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
MSGV1-1D3-28Z.1-3 mut contains an anti-mouse CD19 CAR with CD3 ITAMs mutated.
MSGV1-1D3-28Z.1-3 mut vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells. Blood. 2010 Nov 11;116(19):3875-86.
MSGV1-1D3-28Z.1-3 mut vector Sequence
LOCUS 40924_2079 6941 bp DNA circular SYN 13-MAY-2021 DEFINITION Anti-mouse CD19 CAR with CD3 ITAMs mutated. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6941) AUTHORS Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA TITLE Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells. JOURNAL Blood. 2010 Nov 11;116(19):3875-86. doi: 10.1182/blood-2010-01-265041. Epub 2010 Jul 14. PUBMED 20631379 REFERENCE 2 (bases 1 to 6941) TITLE Direct Submission REFERENCE 3 (bases 1 to 6941) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1182/blood-2010-01-265041"; journalName: "Blood"; date: "2010-11-11- 11"; volume: "116"; issue: "19"; pages: "3875-86" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..6941 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 1..517 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" CDS 1020..1427 /codon_start=1 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" /translation="VTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTFNVG WPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPP LPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA" misc_feature 1437..1807 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" regulatory 1822..1831 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" LTR 3326..3840 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" protein_bind complement(4012..4028) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4036..4066) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4081..4102) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4219..4236) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4390..4978) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5152..6009) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6010..6114) /label=AmpR promoter primer_bind 6182..6200 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(6238..6260) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 6360..6379 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6573..6595 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6723..6742 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer"