MSCB-hDot1Lwt vector (V008318)

Basic Vector Information

      • Vector Name:
      • MSCB-hDot1Lwt
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 9994 bp
      • Type:
      • Retroviral

MSCB-hDot1Lwt vector Vector Map

MSCB-hDot1Lwt9994 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600gag (truncated)HAPGK promoter3' LTRlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterCMV enhancerCMV promoterMMLV Psi

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MSCB-hDot1Lwt vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                9994 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  For packaging hDot1Lwt to retrovirus..
ACCESSION   .
VERSION     .
KEYWORDS    MSCB-hDot1Lwt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Okada Y, Feng Q, Lin Y, Jiang Q, Li Y, Coffield VM, Su L, Xu G, 
            Zhang Y
  TITLE     hDOT1L links histone methylation to leukemogenesis.
  JOURNAL   Cell. 2005 Apr 22;121(2):167-78.
  PUBMED    15851025
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2005-04-22"; volume: "121(2)"; pages: "167-78"
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             428..454
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5344..5843
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5379..5402)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5776..5795
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     LTR             5914..6428
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6590..6606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6614..6644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6659..6680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6797..6814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6968..7556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7048..7067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7727..8587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8350..8369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8588..8692)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8849..9152
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9153..9356
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9307..9327
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    9595..9794
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"

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