Basic Vector Information
- Vector Name:
- pCMV6-XL4
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4707 bp
- Type:
- Mammalian Cell Expression Vectors
- Replication origin:
- ori
- Source/Author:
- Li X.
- Copy Number:
- High copy number
- Promoter:
- CMV
- Cloning Method:
- Enzyme digestion and ligation
- 5' Primer:
- v1.5: GGACTT TCCAAA ATG TCG
- 3' Primer:
- XL39: ATTAGGACAAGGCTGGTGGG
pCMV6-XL4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCMV6-XL4 vector Sequence
LOCUS 40924_12325 4707 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector PCMV6-XL4, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4707)
AUTHORS Li X.
TITLE Direct Submission
JOURNAL Submitted (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct.,
Rockville, MD 20850, USA
REFERENCE 2 (bases 1 to 4707)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 4707)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., Rockville, MD
20850, USA"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4707
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 166..182
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
enhancer 343..722
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 723..926
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 952..970
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 972..1030
/label=multiple cloning site
/note="multiple cloning site"
primer_bind 1031..1048
/label=M13 reverse primer
/note="M13 reverse primer"
primer_bind complement(1032..1048)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 1062..1684
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
promoter 1713..2042
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter complement(2081..2099)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2113..2129)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2137..2153)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2161..2191)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(2206..2227)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2515..3103)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3277..4134)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4135..4239)
/label=AmpR promoter
rep_origin 4266..4694
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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