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PCMV6-XL4 vector (V008374#)

Basic Vector Information

Basic Vector Information
Vector Name PCMV6-XL4 Antibiotic Resistance Ampicillin
Length 4707 bp Type Cloning vector
Source Li X.

PCMV6-XL4 vector Vector Map

M13 fwd CMV T7 promoter multiple cloning site M13 reverse primer hGH poly(A) signal SV40 promoter T7 promoter M13 rev lac operator lac promoter CAP binding site ColE1 ampicillin resistance protein AmpR promoter f1 ori

PCMV6-XL4 vector Sequence

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LOCUS       Exported                4707 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector PCMV6-XL4, complete sequence.
ACCESSION   AF067196
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Li X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., 
            Rockville, MD 20850, USA
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Cloning vector PCMV6-XL4"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:77618"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      201..926
                     /regulatory_class="promoter"
                     /note="CMV"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      953..971
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     misc_feature    972..1030
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     1031..1048
                     /label=M13 reverse primer
                     /note="M13 reverse primer"
     primer_bind     complement(1032..1048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1062..1684
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     regulatory      1063..1684
                     /regulatory_class="polyA_signal_sequence"
                     /note="human growth hormone"
     promoter        1713..2042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1740..2047
                     /label=SV40 origin
                     /note="SV40 origin"
     rep_origin      1893..2028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2081..2099)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2113..2129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2137..2153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2161..2191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2206..2227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2457..3129
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(2515..3103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3274..4134)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AAC24837.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4135..4239)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4266..4694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      4267..4679
                     /label=f1
                     /note="f1"

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