pCMH396 vector (V008419)

LOCUS       40924_11441        9404 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMH396, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Strive T, Hardy CM, Wright J, Reubel GH.
  TITLE     A virus vector based on Canine Herpesvirus for vaccine applications 
            in canids
  JOURNAL   Vet. Microbiol. 119 (2-4), 173-183 (2007)
  PUBMED    17079096
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   Strive T, Wright J, French N, Hardy CM, Reubel GH.
  TITLE     Construction of an infectious Canine herpesvirus bacterial 
            artificial chromosome
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9404)
  AUTHORS   Hardy CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2004) Sustainable Ecosystems, CSIRO, GPO Box 284, 
            Canberra, ACT 2601, Australia
REFERENCE   4  (bases 1 to 9404)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vet. 
            Microbiol. 119 (2-4), 173-183 (2007)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (29-SEP-2004) Sustainable Ecosystems, CSIRO, GPO Box 284, Canberra, 
            ACT 2601, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..6417
                     /isolate="greyhound"
                     /mol_type="other DNA"
                     /country="Australia"
                     /db_xref="taxon:170325"
                     /organism="Canid herpesvirus 1"
     misc_feature    1..36
                     /label=similar to UL19
                     /note="similar to UL19"
     CDS             complement(148..846)
                     /codon_start=1
                     /product="UL20"
                     /label=UL20
                     /protein_id="AAX18917.1"
                     /translation="MDNNHRNNGGETSIKLNNINEIETEEDIYNYISMSSYGGDTDFLV
                     SSAYIVPKTTNQPVFTIYVIFFIISAFVIKPLCCLLFFNYYRATGISEFYNYRWVLLHL
                     YIMERFNFNDMFYLLKPLKVDRLPLLSWQQFLIGLIVLGRTIAFIVIFHSAIFVDSELF
                     FRVSAPVTDSDYITPLIAHKIIPLLSIKVVAFLLIISSAIYTADAICDAIGFIAPRLWV
                     CILMRSYLSF"
     CDS             1123..2691
                     /codon_start=1
                     /product="UL21"
                     /label=UL21
                     /protein_id="AAX18918.1"
                     /translation="MDFKYNDTIVYKGVVFYIYDNGNRAYFTNGGCILSVPRPKNGKDL
                     EIAKFGLTIRGLTSSDRVVANYVKSELNNTGRGWAIPQGEDVFLDSLDLLTHGPGTTER
                     DLCGNLELEIYDTYLTECMVSLKVSSGIIITTGRDLPQERILRLFYEPIITNATSGFVY
                     KPSSTCFALVQAYLTELPESLKSLLFGLFDEIPIQKLSINDNEENSTNVIVTSNKAIST
                     IAKTSSLCYETLRRETIISNFVQVRYIPKPCSIWDPQIKASNLNSIQILSLIFNYIDYV
                     IKSENWNGLDEELNEARFTISVATASIFGENENIYQFIGSNLISNEISKLQLFVLIQFI
                     FARCNLFNCYATLEKLTNSYIQVNQHPIQKLNVNIILDSANEIIRKSSILGNMSILILH
                     LNLPGMIGYKKFTTNIESDSEILTSFVMELENKNRQFMSMEKIDRISFEISKILECLYT
                     GKDIWDCGVALSRSTKNSIPFHIALENKYVSAFDTNISVMRGAYFLHKLVSKRFQKERI
                     NMSIK"
     misc_feature    2835..2860
                     /note="NotI cloning site for insertion of transgenes;
                     replaces non-coding sequence"
     CDS             complement(2903..5293)
                     /codon_start=1
                     /product="UL22"
                     /label=UL22
                     /note="glycoprotein H"
                     /protein_id="AAX18919.1"
                     /translation="MEVKYNFITFIFVIFFMLVFSNDLDNGEYKNLSLSNDFSYSSNVN
                     ITTIENLFSYNNKFVFFFIFNKDSKRENGNLFLFPNFIFKSNKTENNYNIKSQPSEKQL
                     TVFFNPYINLYLSSKYLIDFGVIPESSIQDWYFPRSIQTSTEQNPFGFILSPKRHVPNN
                     GFFNKHKNLYKDGLLNDIMYVNLNSFSIPRKTYIPIYESTWPFDISLFESTLIQNENFC
                     INITIGKEFMGMSITSSKYPPLEMITTPHDANVEMITRYKASMMLDPPGPSEGPLYKVF
                     IIGYGTSKTDSNMYKIMQTIASYPEESLDYRYHLSMANFEVCFLLTYERKFLTNKMDME
                     YFYKIFSRLSTALFSLSEMVRLSKYILNDEIIDIDFNINILTNIIVNHKMFKLSKQLIT
                     SSQVNILLESSRENIVNNLNTFENYNLIFKNDELKIKYLKAVYTYLEIPTEKSLIISRD
                     IINTLYNNSLYETIDWNVTSRKSLFLATSLLLLKTQNIVELRKIILQCTSMCTSDHAIS
                     IEWSLENILNAKNNFKKQFSITDMFSPCMTSTRYDLIEDLHIVDLISVIPNNGNFKNLF
                     SMGHKAASVVNSNILFGSNKIKMLIPELFICKIDINFKFLAILPLNNNNSYIITRKILN
                     RGLTYNVNGIDIANPIFISYINSSNCLYSSVEILPLNLINPKNSKECLYCGSVIMRYLS
                     SGVIIDLLYINDKEAETQLAAGVNSTIPSFNPLSYPVNLKTLLLFPNGTIVTINSITSH
                     EQIKFSNTFIVISIVGIIITSFVVYGIFKMLCSFSYVKYSLLK"
     CDS             complement(5403..6389)
                     /codon_start=1
                     /product="UL23"
                     /label=UL23
                     /note="thymidine kinase"
                     /protein_id="AAX18920.1"
                     /translation="MEVKICRFYIDGCYGTGKSTAALNFIKLEKNNPVYYFPEPMAFWR
                     IILETDIVQGIYSVQDRKLRGELSLNDASLITAQLQTKFSTPYILLHSNVSKFFGENVT
                     FGIPEVIFIFDRHPIASIVCFPLARYLIGDMTLESVISIITTLPKETPGGNLVITDLSE
                     DELLQRLKKRSRKGEKIDLNLLHALQNVYTMLINTKRFLELNDWYKEWDKLPVFNDCDK
                     NYFLSQFCLEKDKPSINDTLFRVFKTSSLVKKNRSPFIIYKWALEKLVDSLENLKIFKV
                     SFQGSPDVCVSNIREIIPHMTITSTSKRFLQNLIKTSKSFSKEMYCD"
     promoter        complement(6468..6486)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6504..6520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6528..6544)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6552..6582)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6597..6618)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6906..7494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7668..8525)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8526..8630)
                     /label=AmpR promoter
     rep_origin      complement(8711..9166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9307..9323
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9330..9348
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9379..9388
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"