pCMH148 vector (V008421)

LOCUS       40924_11431        3734 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCMH148, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Hardy CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2002) Wildlife, Pests and Diseases, CSIRO 
            Sustainable Ecosystems, GPO Box 284, Canberra, ACT 2601, Australia
REFERENCE   2  (bases 1 to 3734)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2002) Wildlife, Pests and Diseases, CSIRO Sustainable 
            Ecosystems, GPO Box 284, Canberra, ACT 2601, Australia"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        40..343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        344..547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          685..781
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    835..858
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    complement(860..994)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1118..1134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1142..1158)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1166..1196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1211..1232)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1520..2108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2282..3139)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3140..3244)
                     /label=AmpR promoter
     primer_bind     3718..3734
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"