pCM130 vector (V008444)

LOCUS       V008444                 7965 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008444
VERSION     V008444
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Marx CJ, Lidstrom ME.
  TITLE     Development of improved versatile broad-host-range vectors for use
            in methylotrophs and other Gram-negative bacteria
  JOURNAL   Microbiology 147 (Pt 8), 2065-2075 (2001)
   PUBMED   11495985
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Marx CJ, Lidstrom ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2000) Microbiology, University of Washington, Box
            357242, Seattle, WA 98195, USA
REFERENCE   3  (bases 1 to 7965)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7965)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology 147 (Pt 8), 2065-2075 (2001)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-DEC-2000) Microbiology, University of Washington, Box 357242,
            Seattle, WA 98195, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label="oriV"
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1773..2693)
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
     misc_feature    complement(2708..2763)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      complement(2865..2892)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(2984..3070)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(3252..3268)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3713..4354)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             4460..5656
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             complement(5893..7038)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(7310..7681)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label="traJ"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             complement(7565..7681)
                     /codon_start=1
                     /gene="traJ'"
                     /product="TraJ'"
                     /label="traJ'"
                     /note="truncated oriT-recognizing protein"
                     /protein_id="AAK73422.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL"
     gene            complement(7565..7681)
                     /gene="traJ'"
                     /label="traJ'"
     oriT            complement(7714..7823)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"