pCHIK-LR 5`GFP vector (V008522)

LOCUS       V008522                14616 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008522
VERSION     V008522
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14616)
  AUTHORS   Tsetsarkin K, Higgs S, McGee CE, De Lamballerie X, Charrel RN,
            Vanlandingham DL.
  TITLE     Infectious clones of Chikungunya virus (La Reunion isolate) for
            vector competence studies
  JOURNAL   Vector Borne Zoonotic Dis. 6 (4), 325-337 (2006)
   PUBMED   17187566
REFERENCE   2  (bases 1 to 14616)
  AUTHORS   Tsetsarkin KA, Vanlandingham DL, Higgs S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) Pathology, University of Texas Medical
            Branch, University Blvd, Galveston, TX 77555-0609, USA
REFERENCE   3  (bases 1 to 14616)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14616)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vector
            Borne Zoonotic Dis."; date: "2006"; volume: "6"; issue: "4"; pages:
            "325-337"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-OCT-2007) Pathology, University of Texas Medical Branch,
            University Blvd, Galveston, TX 77555-0609, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..76
     CDS             77..7498
                     /note="Polyprotein P1234 from Chikungunya virus (strain
                     S27-African prototype). Accession#: Q8JUX6"
                     /label="Polyprotein P1234"
     CDS             7569..8285
                     /label="EGFP"
                     /note="enhanced GFP"
     regulatory      8356..8375
                     /label="second subgenomic promoter"
                     /note="second subgenomic promoter"
                     /regulatory_class="promoter"
     CDS             8443..12186
                     /note="Structural polyprotein from Chikungunya virus
                     (strain S27-African prototype). Accession#: Q8JUX5"
                     /label="Structural polyprotein"
     3'UTR           12190..12687
     promoter        12787..12891
                     /label="AmpR promoter"
     CDS             12892..13749
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13923..14511
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        14599..14616
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"