Basic Vector Information
- Vector Name:
- pmGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6108 bp
- Type:
- Mammalian Expression
- Replication origin:
- ori
- Cloning Method:
- Restriction Enzyme
pmGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pmGFP vector Sequence
LOCUS 40924_30785 6108 bp DNA circular SYN 13-MAY-2021 DEFINITION Control plasmid expressing myc-GFP.. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6108) AUTHORS Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K TITLE Differential Binding of Three Major Human ADAR Isoforms to Coding and Long Non-Coding Transcripts. JOURNAL Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 10.3390/genes8020068. PUBMED 28208661 REFERENCE 2 (bases 1 to 6108) TITLE Direct Submission REFERENCE 3 (bases 1 to 6108) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genes (Basel)."; date: "2017-02-11"; pages: " 10.3390/genes8020068" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..6108 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 123..502 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 503..706 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 751..769 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 797..826 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 836..1552 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" polyA_signal 1593..1817 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1863..2291 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2305..2634 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2701..3492 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3669..3802 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3839..3855) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3863..3879) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3887..3917) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3932..3953) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4070..4087) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4241..4829) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5003..5860) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5861..5965) /label=AmpR promoter primer_bind complement(6040..6059) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker"
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