pEG202 (pLexA) vector (V007602)

LOCUS       40924_17114       10166 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEG202 (pLexA), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Golemis E, Gyuris J, Brent R.
  TITLE     Interaction trap/two-hybrid systems to identify interacting proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Golemis E, Gyuris J, Brent R.
  TITLE     pLexA (pEG202) complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10166)
  AUTHORS   Golemis E, Gyuris J, Brent R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   4  (bases 1 to 10166)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 10166)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East Meadow Circle, 
            Palo Alto, CA 94303-4230, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order
            call (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor. For technical 
            information, call (415) 424-8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. If you suspect there is an 
            error in this sequence, please contact CLONTECH's Technical Support 
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..10166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        807..1511
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1538..2143
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
     regulatory      2165..2174
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      2209..2396
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      3128..4470
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6117..6773)
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     promoter        complement(6774..6961)
                     /label=HIS3 promoter
     promoter        8026..8130
                     /label=AmpR promoter
     CDS             8131..8988
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9162..9750
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9936..10076)
                     /label=bom
                     /note="basis of mobility region from pBR322"