EGFR L858R vector (V012084)

MSDS

Basic Vector Information

Vector Name:: EGFR L858R

Antibiotic Resistance:: Ampicillin

Length:: 8725 bp

Type:: Mammalian Expression, Retroviral

Replication origin:: ori

Selection Marker:: Puromycin

Copy Number:: High Copy

Cloning Method:: Restriction Enzyme

5' Primer:: pBABE5'

EGFR L858R vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

EGFR L858R vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       EGFR_L858R.        8725 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Retroviral construct for expressing human EGFR with L858R mutation 
            in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    EGFR L858R.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, 
            Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M
  TITLE     Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR 
            Mutants.
  JOURNAL   PLoS Med. 2005 Oct 4. 2(11):e313.
  PUBMED    16187797
REFERENCE   2  (bases 1 to 8725)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8725)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Med. 
            2005 Oct 4. 2(11):e313."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..3630
                     /label=EGFR
                     /note="precursor of human epidermal growth factor receptor"
     promoter        3675..4004
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4014..4610
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             4712..5303
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(5433..5450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5604..6192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6355..7212)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7213..7317)
                     /label=AmpR promoter
     LTR             7348..7817
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    7882..8081
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             8282..8698
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"

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