EGFR L858R vector (V012084)

Basic Vector Information

      • Vector Name:
      • EGFR L858R
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 8725 bp
      • Type:
      • Mammalian Expression, Retroviral
      • Replication origin:
      • ori
      • Selection Marker:
      • Puromycin
      • Copy Number:
      • High Copy
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • pBABE5'

EGFR L858R vector Vector Map

EGFR L858R8725 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400EGFRSV40 promoterPuroRLTRL4440oriAmpRAmpR promoterlong terminal repeat from Moloney murine leukemia virusMMLV Psigag (truncated)

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

EGFR L858R vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       EGFR_L858R.        8725 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Retroviral construct for expressing human EGFR with L858R mutation 
            in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    EGFR L858R.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, 
            Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M
  TITLE     Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR 
            Mutants.
  JOURNAL   PLoS Med. 2005 Oct 4. 2(11):e313.
  PUBMED    16187797
REFERENCE   2  (bases 1 to 8725)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8725)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Med. 
            2005 Oct 4. 2(11):e313."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..3630
                     /label=EGFR
                     /note="precursor of human epidermal growth factor receptor"
     promoter        3675..4004
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4014..4610
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             4712..5303
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(5433..5450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5604..6192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6355..7212)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7213..7317)
                     /label=AmpR promoter
     LTR             7348..7817
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    7882..8081
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             8282..8698
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"

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