pEF-FS-EGFP-dneo vector (V007637)

LOCUS       40924_16865        5568 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEF-FS-EGFP-dneo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Wilke S, Gossen M, Wirth D, Buessow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2011) Structural Biology, Helmholtz Centre for 
            Infection Research, Inhoffenstr. 7, Braunschweig 38124, Germany
REFERENCE   2  (bases 1 to 5568)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5568)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2011) Structural Biology, Helmholtz Centre for Infection 
            Research, Inhoffenstr. 7, Braunschweig 38124, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        111..1300
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_recomb     1322..1369
                     /note="3 site; synthetic variant 3 of the yeast
                     flp-recombinase target (FRT)"
     CDS             1388..2104
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2247..2368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2403..2450
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2452..3237
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     promoter        complement(3289..3307)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3333..3557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3816..4404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4578..5435)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5436..5540)
                     /label=AmpR promoter