pEF-F3-EGFP-F3-mcs vector (V007638)

LOCUS       40924_16860        4766 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEF-F3-EGFP-F3-mcs, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Kaufman WL, Kocman I, Agrawal V, Rahn HP, Besser D, Gossen M.
  TITLE     Homogeneity and persistence of transgene expression by omitting 
            antibiotic selection in cell line isolation
  JOURNAL   Nucleic Acids Res. 36 (17), E111 (2008)
  PUBMED    18682524
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Kaufman WL, Gossen M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2010) Structural Biology, Helmholtz Centre for 
            Infection Research, Inhoffenstr. 7, Braunschweig 38124, Germany
REFERENCE   3  (bases 1 to 4766)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2008"; volume: "36"; issue: "17"; pages: "E111"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAR-2010) Structural Biology, Helmholtz Centre for Infection 
            Research, Inhoffenstr. 7, Braunschweig 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        111..1300
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_recomb     1322..1369
                     /note="FRT-3 site; mutant 3 of the flp-recombinase target
                     (FRT), the target site of yeast flp recombinase (flippase)"
     CDS             1388..2104
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2247..2368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     2375..2422
                     /note="FRT-3 site; mutant 3 of the flp-recombinase target
                     (FRT), the target site of yeast flp recombinase (flippase)"
     primer_bind     complement(2436..2452)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2487..2505)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2531..2755
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3014..3602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3776..4633)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4634..4738)
                     /label=AmpR promoter