pEDMS1 vector (V007644)

LOCUS       40924_16820        7909 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pEDMS1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Yamashiro D, Minouchi Y, Ashiuchi M.
  TITLE     Moonlighting Role of a Poly-{gamma}-Glutamate Synthetase Component 
            from Bacillus subtilis: Insight into Novel Extrachromosomal DNA 
            Maintenance
  JOURNAL   Appl. Environ. Microbiol. 77 (8), 2796-2798 (2011)
  PUBMED    21357437
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Ashiuchi M, Yamashiro D.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2010) Contact:Makoto Ashiuchi Kochi University, 
            Agricultural Science, Graduate School of Integrated Arts and 
            Sciences; Monobe 200, Nankoku, Kochi 783-8502, Japan
REFERENCE   3  (bases 1 to 7909)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7909)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2011"; volume: "77"; issue: "8"; pages:
            "2796-2798"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUL-2010) Contact:Makoto Ashiuchi Kochi University, Agricultural
            Science, Graduate School of Integrated Arts and Sciences; Monobe 
            200, Nankoku, Kochi 783-8502, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             37..204
                     /codon_start=1
                     /gene="edmS"
                     /gene_synonym="pgsE"
                     /product="extra-chromosomal DNA-maintenance factor EdmS"
                     /label=edmS
                     /protein_id="BAK19406.1"
                     /translation="MKFVKAIWPFVAVAIVFMFMSAFKFNDQLTDQEKQKIDMEMNKIQ
                     QQEEPVNANK"
     gene            37..204
                     /gene="edmS"
                     /gene_synonym="pgsE"
                     /label=edmS
     rep_origin      complement(749..1337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1511..2368)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2369..2473)
                     /label=AmpR promoter
     CDS             complement(2640..4013)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(4216..5217)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     rep_origin      5368..5395
                     /label=Bacillus subtilis replication origin pBC16: oriU
                     /note="Bacillus subtilis replication origin pBC16: oriU"
     promoter        5642..5670
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"