Basic Vector Information
- Vector Name:
- pECJ3
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5887 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Cameron DE, Collins JJ.
- Promoter:
- PLtetO-1
pECJ3 vector Map
pECJ3 vector Sequence
LOCUS 40924_16790 5887 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pECJ3, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5887)
AUTHORS Cameron DE, Collins JJ.
TITLE Tunable protein degradation in bacteria
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5887)
AUTHORS Cameron DE, Collins JJ.
TITLE Direct Submission
JOURNAL Submitted (12-SEP-2014) Biomedical Engineering, Boston University,
44 Cummington St., Boston, MA 02215, USA
REFERENCE 3 (bases 1 to 5887)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5887)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-SEP-2014) Biomedical Engineering, Boston University, 44
Cummington St., Boston, MA 02215, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5887
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 49..54
/label=PvuII
/note="PvuII"
CDS complement(58..771)
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
misc_feature 772..777
/label=ApaLI
/note="ApaLI"
misc_feature 817..822
/label=SphI
/note="SphI"
regulatory complement(823..896)
/label=PLtetO promoter
/note="PLtetO promoter"
/regulatory_class="promoter"
promoter complement(823..896)
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
misc_feature 842
/label=transcription start
/note="transcription start"
protein_bind 853..871
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 878..896
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
misc_feature 897..902
/label=AclI
/note="AclI"
misc_feature 933..938
/label=SacII
/note="SacII"
misc_feature complement(939..1022)
/label=pdt#3
/note="pdt#3"
CDS complement(1023..2102)
/label=lacI
/note="lac repressor"
misc_feature 2112..2117
/label=BsrGI
/note="BsrGI"
misc_feature 2142..2147
/label=BamHI
/note="BamHI"
promoter complement(2148..2221)
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
misc_feature 2222..2227
/label=EagI
/note="EagI"
misc_feature 2234..2239
/label=NcoI
/note="NcoI"
promoter 2253..2282
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
misc_feature 2288
/label=transcription start
/note="transcription start"
protein_bind 2290..2306
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 2315..2320
/label=SalI
/note="SalI"
regulatory 2337..2340
/label=RBS8
/note="RBS8"
/regulatory_class="ribosome_binding_site"
misc_feature 2347..2352
/label=NheI
/note="NheI"
CDS 2353..2973
/label=TetR
/note="tetracycline repressor TetR"
misc_feature 2977..2982
/label=SacI
/note="SacI"
misc_feature 3001..3006
/label=NdeI
/note="NdeI"
misc_feature 3013..3018
/label=XmaI
/note="XmaI"
regulatory 3019..3093
/label=Ptrc-2 promoter
/note="Ptrc-2 promoter"
/regulatory_class="promoter"
promoter 3032..3061
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 3069..3085
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 3069
/label=transcription start
/note="transcription start"
misc_feature 3094..3099
/label=MfeI
/note="MfeI"
misc_feature 3129..3134
/label=SfoI
/note="SfoI"
CDS 3136..3843
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
misc_feature 3847..3852
/label=EcoRV
/note="EcoRV"
misc_feature 3859..3864
/label=AvrII
/note="AvrII"
misc_feature 3871..3876
/label=XhoI
/note="XhoI"
terminator 3906..3992
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(4156..4744)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(4832..4926)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(4960..5751)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
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