pECGMC3 vector (V007653)

LOCUS       V007653                 9286 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007653
VERSION     V007653
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Cameron DE, Collins JJ.
  TITLE     Tunable protein degradation in bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9286)
  AUTHORS   Cameron DE, Collins JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2014) Biomedical Engineering, Boston University,
            44 Cummington St., Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 9286)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9286)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-SEP-2014) Biomedical Engineering, Boston University, 44
            Cummington St., Boston, MA 02215, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..276
                     /label="P32 promoter"
                     /note="P32 promoter"
                     /regulatory_class="promoter"
     regulatory      226..231
                     /label="-35 binding site"
                     /note="-35 binding site"
                     /regulatory_class="minus_35_signal"
     regulatory      249..254
                     /label="-10 binding site"
                     /note="-10 binding site"
                     /regulatory_class="minus_10_signal"
     CDS             304..1011
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1012..1095
                     /label="pdt#3"
                     /note="pdt#3"
     terminator      1119..1205
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1370..1958)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2046..2140)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2173..3030)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3031..3135)
                     /label="AmpR promoter"
     rep_origin      complement(3167..5714)
                     /direction=LEFT
                     /label="AMB1"
                     /note="AMB1"
     CDS             complement(5735..6469)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     terminator      complement(6655..6682)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(6723..9086)
                     /codon_start=1
                     /product="mf-lon"
                     /label="mf-lon"
                     /protein_id="AIU94531.1"
                     /translation="MSKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNN
                     QMIAIPQIDASVEKPEFSDLHEFGILIDFEVIKEWKDNSLTISTNPIQRCKVISFFENE
                     DQVPYAEVELIESINDFSDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLSLAF
                     DSIMFKLAPSKILTNPEYITSPSLKTRWSIIEKIIFAEDGIITRNAESIDAARQKNEIE
                     QELNHKLKEKMDKQQKEYYLREKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVK
                     RKIMASIKRVEALQSGTPEWNTEKNYIDWMMSIPWWEETEDLTDLKYAKKILDKHHYGM
                     KKVKERIIEYLAVKTKTKSLKAPIITLVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVK
                     DESEIRGHRKTYVGSMPGRIIQTMKRAKVKNPLFLLDEIDKMASDHRGDPASAMLEVLD
                     PEQNKEFSDHYIEEPYDLSQVMFIATANYPEDIPEALYDRMEIINLSSYTEIEKVKIAQ
                     DYLVPKAIEQHELTSEEISFTEGAINEIIKYYTREAGVRQLERHINSIIRKYIVKNLNG
                     EMDKIVIDEKQVNDLLGKRIFDHTEKQEESQIGVVTGLAYTQFGGDILPIEVSLYPGKG
                     NLILTGKLGEVMKESATIALTYVKSNFEKFGVDKKVFEENDIHVHVPEGAVPKDGPSAG
                     ITITTALISALSDKPVSKEIGMTGEITLRGNVLPIGGLREKSISASRSGLKTIIIPKKN
                     ERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKTK"
     regulatory      complement(9105..9280)
                     /label="nisA promoter"
                     /note="nisA promoter"
                     /regulatory_class="promoter"
     regulatory      complement(9132..9137)
                     /label="-10 region"
                     /note="-10 region"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(9158..9163)
                     /label="-35 region"
                     /note="-35 region"
                     /regulatory_class="minus_35_signal"