pEBM103 vector (V007679)

LOCUS       V007679                11541 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007679
VERSION     V007679
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Lynd LR.
  TITLE     Identification of sporulation histidine kinases in C. thermocellum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Mearls EB.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2013) Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 11541)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2013) Engineering, Dartmouth College, 14 Engineering Dr,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      47..294
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(542..1130)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1217..1795)
                     /codon_start=1
                     /gene="tdk"
                     /product="tdk"
                     /label="tdk"
                     /protein_id="AGO62074.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(1217..1795)
                     /gene="tdk"
                     /label="tdk"
     regulatory      1796..2416
                     /gene="cbp"
                     /regulatory_class="promoter"
     gene            1796..2416
                     /gene="cbp"
                     /label="cbp"
     CDS             complement(2495..3496)
                     /label="repB"
                     /note="RepB replication protein"
     CDS             complement(4057..4914)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(5013..5813)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(5814..6034)
                     /label="URA3 promoter"
     misc_feature    6062..6565
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_recomb     6570..6728
     misc_recomb     7855..8881
     regulatory      8897..9544
                     /gene="gapD"
                     /regulatory_class="promoter"
     gene            8897..9544
                     /gene="gapD"
                     /label="gapD"
     CDS             9545..10192
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             10213..10758
                     /codon_start=1
                     /gene="hpt"
                     /product="hpt"
                     /label="hpt"
                     /note="hypoxanthine phosphoribosyltransferase"
                     /protein_id="AGO62077.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            10213..10758
                     /gene="hpt"
                     /label="hpt"
     misc_recomb     10822..11541