pEAVrVBS vector (V007687)

LOCUS       V007687                14553 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007687
VERSION     V007687
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Balasuriya UB, Snijder EJ, Heidner HW, Zhang J, Zevenhoven-Dobbe JC,
            Boone JD, McCollum WH, Timoney PJ, Maclachlan NJ.
  TITLE     Development and characterization of an infectious cDNA clone of the
            virulent Bucyrus strain of Equine arteritis virus
  JOURNAL   J. Gen. Virol. 88 (PT 3), 918-924 (2007)
   PUBMED   17325365
REFERENCE   2  (bases 1 to 14553)
  AUTHORS   Balasuriya UB, MacLachlan J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2006) Veterinary Science, University of Kentucky,
            108 Gluck Equine Research Center, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14553)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14553)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Gen.
            Virol. 88 (PT 3), 918-924 (2007)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2006) Veterinary Science, University of Kentucky, 108 Gluck
            Equine Research Center, Lexington, KY 40546, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             225..5399
                     /codon_start=1
                     /product="replicase polyprotein"
                     /label="replicase polyprotein"
                     /note="ORF1a"
                     /protein_id="ABI64080.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYASAWVASPAANSLIVTIDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFDNVRVVTPEGQPRPVPAP
                     RVRPSANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPLKTPAVQRYTMTLKMMRS
                     RFSWHCDVWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNSVVLTALLVSSANYVASM
                     DHQCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCRNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VTKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTMLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSNDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQ"
     misc_feature    5399..5405
                     /note="site of ribosomal frameshift; slippery sequence"
     CDS             5405..9751
                     /codon_start=1
                     /product="replicase polyprotein"
                     /label="replicase polyprotein"
                     /note="ORF1b; translated from ORF1a by programmed -1
                     ribosomal frameshift"
                     /protein_id="ABI64088.1"
                     /translation="LRAPHIFPGDVGRRTFADSKDKGFVALHSRTMFLAARDFLFNIKF
                     VCDEEFTKTPKDTLLGYVRACPGYWFIFRRTHRSLIDAYWDSMECVYALPTISDFDVSP
                     GDVAVTGERWDFESPGGGRAKRLTADLVHAFQGFHGASYSYDDKVAAAVSGDPYRSDGV
                     LYNTRWGNIPYSVPTNALEATACYRAGCEAVTDGTNVIATIGPFPEQQPIPDIPKSVLD
                     NCADISCDAFIAPAAETALCGDLEKYNLSTQGFVLPSVFSMVRAYLKEEIGDAPPLYLP
                     STVPSKNSQAGINGAEFPTKSLQSYCLIDDMVSQSMKSNLQTATMATCKRQYCSKYKIR
                     SILGTNNYIGLGLRACLSGVTAAFQKAGKDGSPIYLGKSKFDPIPAPDKYCLETDLESC
                     DRSTPALVRWFATNLIFELAGQPELVHSYVLNCCHDLVVAGSVAFTKRGGLSSGDPITS
                     ISNTIYSLVLYTQHMLLCGLEGYFPEIAEKYLDGSLELRDMFKYVRVYIYSDDVVLTTP
                     NQHYAASFDRWVPHLQALLGFKVDPKKTVNTSSPSFLGCRFKQVDGKCYLASLQDRVTR
                     SLLYHIGAKNPSEYYEAAVSIFKDSIICCDEDWWTDLHRRISGAARTDGVEFPTIEMLT
                     SFRTKQYESAVCTVCGAAPVAKSACGGWFCGNCVPYHAGHCHTTSLFANCGHDIMYRST
                     YCTMCEGSPKQMVPKVPHPILDHLLCHIDYGSKEELTLVVADGRTTSPPGRYKVGHKVV
                     AVVADVGGNIVFGCGPGSHIAVPLQDTLKGVVVNKALKNAAASEYVEGPPGSGKTFHLV
                     KDVLAVVGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTVLDFPRPGSGNITVRLP
                     QVGTSEGETFVDEVAYFSPVDLARILTQGRVKGYGDLNQLGCVGPASVPRNLWLRHFVC
                     LEPLRVCHRFGAAVCDLIKGIYPYYEPAPHTTKVVFVPNPDFEKGVVITAYHKDRGLGH
                     RTIDSIQGCTFPVVTLRLPTPQSLTRPRAVVAVTRASQELYIYDPFDQLSGLLKFTKEA
                     EAQDLIHGPPTACHLGQEIDLWSNEGLEYYKEVNLLYTHVPIKDGVIHSYPNCGPACGW
                     EKQSNKISCLPRVAQNLGYHYSPDLPGFCPIPKELAEHWPVVSNDRYPNCLQITLQQVC
                     ELSKPCSAGYMVGQSVFVQTPGVTSYWLTEWVDGKARALPDSLFSSGRFETNSRAFLDE
                     AEEKFAAAHPHACLGEINKSTVGGSHFIFSQYLPPLLPADAVALVGASLAGKAAKAACS
                     VVDVYAPSFEPYLHPETLSRVYKIMIDFKPCRLMVWRNATFYVQEGVDAVTSALAAVSK
                     LIKVPANEPVSFHVASGYRTNALVAPQAKISIGAYAAEWALSTEPPPAGYAIVRRYIVK
                     RLLSSTEVFLCRRGVVSSTSVQTICALEGCKPLFNFLQIGSVIGPV"
     regulatory      6374..6383
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9824..10504
                     /gene="GP2b"
                     /label="Glycoprotein 2b"
                     /note="Glycoprotein 2b from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28992"
     CDS             10700..11155
                     /gene="GP4"
                     /label="Glycoprotein 4"
                     /note="Glycoprotein 4 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28994"
     CDS             11146..11910
                     /gene="GP5"
                     /label="Glycoprotein 5"
                     /note="Glycoprotein 5 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28995"
     CDS             11901..12386
                     /gene="M"
                     /label="Membrane protein"
                     /note="Membrane protein from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28991"
     promoter        12785..12889
                     /label="AmpR promoter"
     CDS             12890..13747
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13921..14509
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"