pE5n vector (V007701)

LOCUS       40924_16435        3030 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pE5n, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G.
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants
  JOURNAL   Plant Methods 4, 3 (2008)
  PUBMED    18211686
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2007) Cell Signalling Laboratory, Stazione 
            Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy
REFERENCE   3  (bases 1 to 3030)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3030)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods 4, 3 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2007) Cell Signalling Laboratory, Stazione Zoologica Anton 
            Dohrn, Villa Comunale, Naples 80121, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    358..457
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             481..1197
                     /codon_start=1
                     /label=ECFP
                     /note="enhanced CFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1201..1232
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    complement(1259..1358)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1481..2287
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2380..2968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"