pDYN6902 vector (V007706)

LOCUS       V007706                10975 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007706
VERSION     V007706
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Thibessard A, Bertrand C, Hiblot J, Piotrowski E, Leblond P.
  TITLE     Construction of pDYN6902, a new Streptomyces integrative expression
            vector designed for cloning sequences interfering with Escherichia
            coli viability
  JOURNAL   Plasmid (2015) In press
   PUBMED   26476355
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   Thibessard A, Bertrand C, Hiblot J, Piotrowski E, Leblond P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2015) Faculte des Sciences et Technologies,
            Universite de Lorraine, Boulevard des Aiguillettes Bp 239,
            Vandoeuvre F-54506, France
REFERENCE   3  (bases 1 to 10975)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10975)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid
            (2015) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUL-2015) Faculte des Sciences et Technologies, Universite de
            Lorraine, Boulevard des Aiguillettes Bp 239, Vandoeuvre F-54506,
            France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      149..197
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     regulatory      190..339
                     /function="transcriptional terminator"
                     /label="terminator fd"
                     /note="terminator fd"
                     /regulatory_class="terminator"
     misc_feature    345..401
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     regulatory      complement(408..515)
                     /function="thiostrepton inducible promoter"
                     /label="pTipA promoter"
                     /note="pTipA promoter"
                     /regulatory_class="promoter"
     terminator      572..606
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(628..644)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(737..1210)
                     /function="involved in the partition system"
                     /label="SopC binding site"
                     /bound_moiety="SopC"
                     /note="sopC is the target site of SopAB complex"
     misc_feature    737..1210
                     /gene="sopC"
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1286..2254)
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(2257..3429)
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(3755..4005)
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4011..4763)
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     rep_origin      complement(4854..5073)
                     /direction=LEFT
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     rep_origin      complement(5092..5158)
                     /direction=LEFT
                     /note="ori2 (oriS) from the F (fertility) factor of E.
                     coli"
     CDS             5457..6257
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             6729..7535
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             complement(7731..8099)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(8132..8241)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(8479..8490)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    8561..8660
                     /label="phage phi-C31 attP"
                     /note="attachment site of phage phi-C31"
     CDS             8670..10484
                     /label="phage phi-C31 integrase"
                     /note="integrase from phage phi-C31"
     primer_bind     10930..10946
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"