pDusk vector (V007720)

LOCUS       V007720                 6220 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007720
VERSION     V007720
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Moglich A.
  TITLE     From dusk till dawn: one-plasmid systems for light-regulated gene
            expression
  JOURNAL   J. Mol. Biol. 416 (4), 534-542 (2012)
   PUBMED   22245580
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Ohlendorf R, Moffat K, Moeglich A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2011) Biophysikalische Chemie, Humboldt
            Universitaet zu Berlin, Invalidenstrasse 42, Berlin 10115, Germany
REFERENCE   3  (bases 1 to 6220)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6220)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol.
            Biol."; date: "2012"; volume: "416"; issue: "4"; pages: "534-542"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-AUG-2011) Biophysikalische Chemie, Humboldt Universitaet zu
            Berlin, Invalidenstrasse 42, Berlin 10115, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(140..157)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     misc_feature    complement(158..203)
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             complement(205..237)
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             complement(241..258)
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             complement(268..285)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     RBS             complement(304..326)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      complement(339..585)
                     /label="FixK2 promoter"
                     /note="FixK2 promoter"
                     /regulatory_class="promoter"
     promoter        880..957
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             958..2100
                     /codon_start=1
                     /product="YF1"
                     /label="YF1"
                     /protein_id="AFH34990.1"
                     /translation="MASFQSFGIPGQLEVIKKALDHVRVGVVITDPALEDNPIVYVNQG
                     FVQMTGYETEEILGKNCRFLQGKHTDPAEVDNIRTALQNKEPVTVQIQNYKKDGTMFWN
                     ELNIDPMEIEDKTYFVGIQNDITEHQQTQARLQELQSELVHVSRLSAMGEMASALAHEL
                     NQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAGQIIRRLRDFVARGES
                     EKRVESLSKLIEEAGALGLAGAREQNVQLRFSLDPGADLVLADRVQIQQVLVNLFRNAL
                     EAMAQSQRRELVVTNTPAADDMIEVEVSDTGSGFQDDVIPNLFQTFFTTKDTGMGVGLS
                     ISRSIIEAHGGRMWAESNASGGATFRFTLPAADEMIGGLA"
     CDS             2097..2711
                     /gene="fixJ"
                     /label="Transcriptional regulatory protein FixJ"
                     /note="Transcriptional regulatory protein FixJ from
                     Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC
                     13528 / IAM 13628 / NBRC 14792 / USDA 110). Accession#:
                     P23221"
     CDS             3515..3703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    3808..3950
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4136..4724)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             4846..5658
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(5754..6209)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"