pDUO-30T vector (V007721)

LOCUS       40924_16310        6022 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Bicistronic shuttle vector pDUO-30T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Nakata PA.
  TITLE     Construction of pDUO: A bicistronic shuttle vector series for dual 
            expression of recombinant proteins
  JOURNAL   Plasmid 89, 16-21 (2016)
  PUBMED    27989736
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Nakata PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2016) Pediatrics, USDA/ARS Children's Nutrition 
            Research Center, Baylor College of Medicine, 1100 Bates Street, 
            Houston, TX 77030-2600, USA
REFERENCE   3  (bases 1 to 6022)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 89,
            16-21 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-AUG-2016) Pediatrics, USDA/ARS Children's Nutrition Research 
            Center, Baylor College of Medicine, 1100 Bates Street, Houston, TX 
            77030-2600, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(733..1263)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1452..1480)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      complement(1521..1872)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             1886..2716
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     terminator      2745..2788
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2990..3034)
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     misc_feature    complement(3041..3099)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     RBS             3117..3139
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    3117..3122
                     /label=RBS
                     /note="RBS"
     promoter        complement(3166..3450)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     misc_feature    3200..3238
                     /label=I2 and I1 region
                     /note="I2 and I1 region"
     misc_feature    3235..3248
                     /label=CAP binding site
                     /note="CAP binding site"
     misc_feature    3269..3290
                     /label=O1 region
                     /note="O1 region"
     misc_feature    3432..3447
                     /label=O2 region
                     /note="O2 region"
     terminator      complement(3456..3502)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3747..3764)
                     /label=6xHis
                     /note="6xHis affinity tag"
     RBS             complement(3788..3810)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(3837..4121)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             4148..5023
                     /label=araC
                     /note="L-arabinose regulatory protein"
     oriT            5183..5291
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(5363..5951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"