pDGO134 vector (V008002)

LOCUS       V008002                 9473 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008002
VERSION     V008002
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Olson DG.
  TITLE     Conversion of phosphoenolpyruvate to pyruvate in Clostridium
            thermocellum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Olson DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 9473)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..1005)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(1006..1460)
                     /direction=LEFT
                     /label="C. thermocellum replication origin"
                     /note="C. thermocellum replication origin"
     CDS             complement(1566..2423)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2424..2528)
                     /label="AmpR promoter"
     CDS             4857..5504
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             5525..6070
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /note="Hpt; counterselection with 8AZH"
                     /protein_id="AOC59192.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            5525..6070
                     /gene="hpt"
                     /label="hpt"
     misc_feature    6255..7043
                     /note="partial pyruvate kinase gene from T.
                     saccharolyticum"
     terminator      7076..7264
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(7524..8112)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8199..8777)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /note="Tdk; counterselection with FUDR"
                     /protein_id="AOC59190.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(8199..8777)
                     /gene="tdk"
                     /label="tdk"