pDGO-68 vector (V008004)

LOCUS       V008004                 7023 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008004
VERSION     V008004
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Olson DG.
  TITLE     Conversion of phosphoenolpyruvate to pyruvate in Clostridium
            thermocellum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Olson DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 7023)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7023)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-FEB-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..455)
                     /direction=LEFT
                     /label="C. thermocellum replication origin"
                     /note="C. thermocellum replication origin"
     CDS             complement(561..1418)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1419..1523)
                     /label="AmpR promoter"
     CDS             2191..2838
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             2859..3404
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /note="Hpt; counterselection with 8AZH"
                     /protein_id="AOC59180.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            2859..3404
                     /gene="hpt"
                     /label="hpt"
     terminator      3621..3809
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(4069..4657)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4744..5322)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /note="Tdk; marker for 5FOA counter selection"
                     /protein_id="AOC59182.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(4744..5322)
                     /gene="tdk"
                     /label="tdk"
     CDS             complement(6022..7023)
                     /label="repB"
                     /note="RepB replication protein"