pDGO-40 vector (V008006)

LOCUS       V008006                12184 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008006
VERSION     V008006
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Olson DG, Giannone RJ, Hettich RL, Lynd LR.
  TITLE     Role of the CipA Scaffoldin Protein in Cellulose Solubilization, as
            Determined by Targeted Gene Deletion and Complementation in
            Clostridium thermocellum
  JOURNAL   J. Bacteriol. 195 (4), 733-739 (2013)
   PUBMED   23204466
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   Olson DG, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2012) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 12184)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2013"; volume: "195"; issue: "4"; pages:
            "733-739"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-OCT-2012) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..573
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      829..1043
                     /label="Ctherm_ori"
                     /note="Ctherm_ori"
     CDS             1044..2045
                     /label="repB"
                     /note="RepB replication protein"
     CDS             2155..2802
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2886..3743)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3744..3835)
                     /label="AmpR promoter"
     terminator      complement(3854..3881)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(3973..4059)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             5087..10645
                     /gene="cipA"
                     /label="Cellulosomal-scaffolding protein A"
                     /note="Cellulosomal-scaffolding protein A from Acetivibrio
                     thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 /
                     NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372).
                     Accession#: Q06851"
     promoter        complement(10748..11032)
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             11059..11934
                     /label="araC"
                     /note="L-arabinose regulatory protein"