pDGO-03 vector (V008009)

LOCUS       V008009                 9045 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008009
VERSION     V008009
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Waller BH, Olson DG, Currie DH, Guss AM, Lynd LR.
  TITLE     Exchange of type II dockerin-containing subunits of the Clostridium
            thermocellum cellulosome as revealed by SNAP-tags
  JOURNAL   FEMS Microbiol. Lett. 338 (1), 46-53 (2013)
   PUBMED   23082914
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   Olson DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2012) Thayer School of Engineering, Dartmouth
            College, Thayer School of Engineering, Dartmouth College, Hanover,
            NH 03755, USA
REFERENCE   3  (bases 1 to 9045)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9045)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "2013"; volume: "338"; issue: "1"; pages:
            "46-53"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-AUG-2012) Thayer School of Engineering, Dartmouth College,
            Thayer School of Engineering, Dartmouth College, Hanover, NH 03755,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(906..1706)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1707..1927)
                     /label="URA3 promoter"
     misc_feature    1955..2458
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_feature    complement(2503..3041)
                     /label="3' flank"
                     /note="3' flank"
     misc_feature    complement(3042..3583)
                     /label="5' flank"
                     /note="5' flank"
     regulatory      complement(3589..3720)
                     /label="hpt"
                     /note="hpt"
                     /regulatory_class="terminator"
     CDS             complement(3721..4275)
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /protein_id="AFU51881.1"
                     /translation="MINQIKEILVTREELKNNAKELGKRISSDYEGKELVLIGVLKGGV
                     VFFADLIREITIPIDVDFISVSSYGNSTKSSGVVRIIKDIDIDITNKHVLIVEDLVDTG
                     LTLHYLKSMFEARGPKDVKICTALDKPSRRKVDLEIDYKGITIPDKFVVGYGLDYAEKY
                     RNLPDVCVLDSSVYTDKEDMD"
     gene            complement(3721..4275)
                     /gene="hpt"
                     /label="hpt"
     CDS             complement(4294..4941)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      complement(4942..5469)
                     /label="gapDH promoter region"
                     /note="gapDH promoter region"
                     /regulatory_class="promoter"
     misc_feature    complement(5473..6040)
                     /label="internal flank"
                     /note="internal flank"
     CDS             complement(6044..6622)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /note="from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AFU51883.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(6044..6622)
                     /gene="tdk"
                     /label="tdk"
     regulatory      complement(6623..7243)
                     /note="CBP; cellulose-binding protein promoter"
                     /regulatory_class="promoter"
     CDS             complement(7353..8354)
                     /label="repB"
                     /note="RepB replication protein"