pDBR vector (V008063)

LOCUS       V008063                 6619 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008063
VERSION     V008063
KEYWORDS    complete replicon
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Calderon-Gomez E, Lampropoulou V, Shen P, Neves P, Roch T, Stervbo
            U, Rutz S, Kuehl AA, Heppner FL, Loddenkemper C, Charneau P,
            Fillatreau S.
  TITLE     Reprogrammed quiescent B cells provide an effective cellular therapy
            against chronic experimental encephalomyelitis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Fillatreau S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2011) Fillatreau S., Immune Regulation, Deutsche
            Rheuma-ForschungsZentrum, a Leibniz Institute, Chariteplatz 1,
            D-10117, GERMANY
REFERENCE   3  (bases 1 to 6619)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6619)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-FEB-2011) Fillatreau S., Immune Regulation, Deutsche
            Rheuma-ForschungsZentrum, a Leibniz Institute, Chariteplatz 1,
            D-10117, GERMANY"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   835..1015
                     /label="HIV 5' LTR"
                     /note="HIV 5' LTR"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1684..1917
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2102..2146
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2295..2336
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2444..2561
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     misc_feature    2657..2690
                     /label="LoxP"
                     /note="LoxP"
     protein_bind    2657..2690
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     enhancer        2806..3109
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3110..3313
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    3345..3396
                     /note="multiple cloning site (BamH1, Xba1, Pme1, Ecor1,
                     Xho1)"
     protein_bind    complement(3419..3452)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3508..4096
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4099..4115)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             4625..4805
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4867..5455)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5629..6486)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6487..6591)
                     /label="AmpR promoter"