pDblet vector (V008064)

LOCUS       40924_14190        6238 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDblet, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Zhu J, Carlson DL, Dubey DD, Sharma K, Huberman JA.
  TITLE     Comparison of the two major ARS elements of the ura4 replication 
            origin region with other ARS elements in the fission yeast, 
            Schizosaccharomyces pombe
  JOURNAL   Chromosoma 103 (6), 414-422 (1994)
  PUBMED    7859562
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Brun C, Dubey DD, Huberman JA.
  TITLE     pDblet, a stable autonomously replicating shuttle vector for 
            Schizosaccharomyces pombe
  JOURNAL   Gene 164 (1), 173-177 (1995)
  PUBMED    7590311
REFERENCE   3  (bases 1 to 6238)
  AUTHORS   Brun C, Dubey DD, Huberman JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2002) Cancer Genetics, Roswell Park Cancer 
REFERENCE   4  (bases 1 to 6238)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6238)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chromosoma"; date: "1994"; volume: "103"; issue: "6"; pages: 
            "414-422"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Gene"; 
            date: "1995"; volume: "164"; issue: "1"; pages: "173-177"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2002) Cancer Genetics, Roswell Park Cancer Institute, Elm "
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4974..6171
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4896"
                     /organism="Schizosaccharomyces pombe"
     CDS             complement(628..1419)
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(2007..2462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2607..2623
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2633..2651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2660..2767)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(2780..2798)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2819..2835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2843..2859)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2867..2897)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2912..2933)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3221..3809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3983..4840)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4841..4945)
                     /label=AmpR promoter
     rep_origin      4974..6171
                     /label=ARS doublet bordered by AatII sites
                     /note="ARS doublet bordered by AatII sites"