2XMyc-LRRK2-WT vector (V012134)

LOCUS       V012134                11985 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V012134
VERSION     V012134
KEYWORDS    2XMyc-LRRK2-WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11985)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
   PUBMED   18397888
REFERENCE   2  (bases 1 to 11985)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11985)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        621..639
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      671..680
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             680..709
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             725..754
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     protein_bind    773..797
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             812..8392
                     /gene="LRRK2"
                     /label="Leucine-rich repeat serine/threonine-protein kinase
                     2"
                     /note="Leucine-rich repeat serine/threonine-protein kinase
                     2 from Homo sapiens. Accession#: Q5S007"
     protein_bind    complement(8414..8438)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(8488..8506)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8780..8901
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8908..9363)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9390..9492
                     /label="AmpR promoter"
     promoter        9496..9853
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             9888..10679
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(10870..10889)
                     /label="TK-pA-R"
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10914..10961
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11290..11878
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"