pBART-SIL6 vector (V009278)

LOCUS       V009278                10830 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009278
VERSION     V009278
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Kooij TW, Rauch MM, Matuschewski K.
  TITLE     Expansion of experimental genetics approaches for Plasmodium berghei
            with versatile transfection vectors
  JOURNAL   Mol. Biochem. Parasitol. 185 (1), 19-26 (2012)
   PUBMED   22705315
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Kooij TWA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2012) Parasitology Unit, Max Planck Institute for
            Infection Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany
REFERENCE   3  (bases 1 to 10830)
  AUTHORS   Kooij TWA.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2012) Parasitology Unit, Max Planck Institute for
            Infection Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany
REFERENCE   4  (bases 1 to 10830)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 10830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Biochem. Parasitol."; date: "2012"; volume: "185"; issue: "1";
            pages: "19-26"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAY-2012) Parasitology Unit, Max Planck Institute for Infection
            Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (29-AUG-2012) Parasitology Unit, Max Planck Institute for Infection
            Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany"
            SGRef: number: 4; type: "Journal Article"
            On Aug 29, 2012 this sequence version replaced JX099568.1.
FEATURES             Location/Qualifiers
     source          1..10830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..18
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        19..37
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    69..558
                     /note="SIL6L; silent intergenic locus on Plasmodium berghei
                     chromosome 6"
     misc_feature    585..638
                     /note="5'MCS; multiple cloning site"
     CDS             672..1376
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1410..1439
                     /label="c-Myc epitope tag"
                     /note="c-Myc epitope tag"
     CDS             1410..1439
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    1446..1475
                     /label="c-Myc epitope tag"
                     /note="c-Myc epitope tag"
     CDS             1446..1475
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    1482..1511
                     /label="c-Myc epitope tag"
                     /note="c-Myc epitope tag"
     CDS             1482..1511
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     3'UTR           1527..1940
                     /note="3'PbPPPK-DHPS; 3'UTR of Plasmodium berghei
                     hydroxymethylpterin pyrophosphokinase-dihydropteroate
                     synthetase"
     5'UTR           1962..3331
                     /note="5'PbHSP70; 5'UTR of Plasmodium berghei heat shock
                     protein 70"
     CDS             3355..4068
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     3'UTR           4084..4613
                     /note="3'PbDHFS-FPGS; 3'UTR of Plasmodium berghei
                     dihydrofolate synthase-folylpolyglutamate synthase"
     3'UTR           complement(4626..5081)
                     /note="3'PbDHFR-TS; 3'UTR of Plasmodium berghei
                     dihydrofolate reductase-thymidylate synthase"
     CDS             complement(5085..5729)
                     /gene="FUR1"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P18562"
     CDS             complement(5730..6200)
                     /gene="FCY1"
                     /label="Cytosine deaminase"
                     /note="Cytosine deaminase from Saccharomyces cerevisiae
                     (strain ATCC 204508 / S288c). Accession#: Q12178"
     CDS             complement(6243..6803)
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     5'UTR           complement(6804..7747)
                     /note="5'PbDHFR-TS; 5'UTR of Plasmodium berghei
                     dihydrofolate reductase-thymidylate synthase"
     misc_feature    7754..8343
                     /note="5'PbHSP70_duplicate; duplicated sequence of 5'UTR of
                     Plasmodium berghei heat shock protein 70"
     misc_feature    8344..8406
                     /note="3'MCS; multiple cloning site"
     misc_feature    8433..8906
                     /note="SIL6R; silent intergenic locus on Plasmodium berghei
                     chromosome 6"
     promoter        complement(8936..8953)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8958..8974)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(9135..9723)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9897..10754)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10755..10826)
                     /label="AmpR promoter"