pBARST vector (V009279)

LOCUS       40924_5914        4977 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBARST DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Construction of pDESTR, a GATEWAY vector for gene disruption in 
            filamentous fungi
  JOURNAL   Curr. Microbiol. 52 (3), 210-215 (2006)
  PUBMED    16479358
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Sone T, Abe A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2005) Teruo Sone, Hokkaido University, Graduate 
            School of Agriculture; Kita 9 Nishi 9, Kita-ku, Sapporo, Hokkaido 
            060-8589, Japan (E-mail:sonet@chem.agr.hokudai.ac.jp, 
            Tel:81-11-706-2502, Fax:81-11-706-4961)
REFERENCE   3  (bases 1 to 4977)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol."; date: "2006"; volume: "52"; issue: "3"; pages: 
            "210-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2005) Teruo Sone, Hokkaido University, Graduate School of 
            Agriculture"; volume: " Kita 9 Nishi 9, Kita-ku, Sapporo, Hokkaido 
            060-8589, Japan (E-mail:sonet@chem.agr.hokudai.ac.jp, 
            Tel:81-11-706-2502, Fax"; pages: "81-11-706-4961"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(233..821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(995..1852)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1853..1957)
                     /label=AmpR promoter
     promoter        2063..2164
                     /label=TRP1 promoter
     promoter        2259..2618
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             2641..3189
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     protein_bind    3270..3394
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3419..3449
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3503..4159
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             4504..4806
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4850..4974)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"