PBAD1030A vector (V009302)

Price Information

Cat No. Plasmid Name Availability Add to cart
V009302 PBAD1030A In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • PBAD1030A
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3586 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Chakravartty V, Cronan JE.
      • Growth Strain(s):
      • Top10
      • Growth Temperature:
      • 37℃

PBAD1030A vector Vector Map

PBAD1030A3586 bp6001200180024003000AraCaraBAD promoterrrnB T1 terminatorrrnB T2 terminatorRSF oriAmpR promoterbla

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

PBAD1030A vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3586 bp DNA     circular SYN 29-NOV-2023
DEFINITION  Cloning vector PBAD1030A, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Chakravartty V, Cronan JE.
  TITLE     A series of medium and high copy number arabinose-inducible 
            Escherichia coli expression vectors compatible with pBR322 and 
  JOURNAL   Plasmid 81, 21-26 (2015)
  PUBMED    26021570
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Chakravartty V, Cronan JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2015) Microbiology, University of Illinois, 601S. 
            Goodwin Ave, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 3586)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3586)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 81,
            21-26 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAR-2015) Microbiology, University of Illinois, 601S. Goodwin 
            Ave, Urbana, IL 61801, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
FEATURES             Location/Qualifiers
     source          1..3586
                     /mol_type="other DNA"
                     /organism="Cloning vector PBAD1030A"
     CDS             complement(96..974)
                     /note="transcription factor"
     gene            complement(96..974)
     promoter        1001..1285
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     terminator      1569..1655
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
     terminator      1747..1774
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
     misc_feature    complement(1834..2462)
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin 
                     ofreplication can be propagated in E. coli cells that 
                     contain additional plasmids with compatible origins."
     promoter        2479..2583
                     /label=AmpR promoter
     CDS             2584..3444
                     /note="ampicillin resistance"
     gene            2584..3444