pBacPAK1 vector (V009312)

LOCUS       40924_5674        5461 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBacPAK1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Kitts PA, Possee RD.
  TITLE     A method for producing recombinant baculovirus expression vectors at
            high frequency
  JOURNAL   BioTechniques 14 (5), 810-817 (1993)
  PUBMED    8512707
REFERENCE   3  (bases 1 to 5461)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 5461)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5461)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1993"; volume: "14"; issue: "5"; pages: 
            "810-817"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This sequence has been compiled from information in the sequence 
            databases, published literature and other sources; this vector has 
            not been completely sequenced. This vector is no longer available 
            from CLONTECH and CLONTECH will not update or revise this sequence.
FEATURES             Location/Qualifiers
     source          1..5461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1158..1249
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     1260..2617
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      2764..3219
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3501..3605
                     /label=AmpR promoter
     CDS             3606..4463
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4637..5225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"