pAQJ4-MCS vector (V009465)

LOCUS       40924_4694        5878 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cyanobacterial shuttle vector pAQJ4-MCS DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Onizuka T, Endo S, Akiyama H, Kanai S, Hirano M, Yokota A, Tanaka S,
            Miyasaka H.
  TITLE     The rbcX gene product promotes the production and assembly of 
            ribulose-1,5-bisphosphate carboxylase/oxygenase of Synechococcus sp.
            PCC7002 in Escherichia coli
  JOURNAL   Plant Cell Physiol. 45 (10), 1390-1395 (2004)
  PUBMED    15564522
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Miyasaka H, Tanaka S, Onizuka T, Akiyama H.
  TITLE     Cyanobacterial shuttle vector pAQJ4-MCS
  JOURNAL   Published Only in Database (2009)
REFERENCE   3  (bases 1 to 5878)
  AUTHORS   Miyasaka H, Tanaka S, Onizuka T, Akiyama H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2009) Contact:Takuo Onizuka Toray Research Center,
            Inc., Biological Science Laboratories; 1111 Tebiro, Kamakura, 
            Kanagawa 248-0036, Japan
REFERENCE   4  (bases 1 to 5878)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol."; date: "2004"; volume: "45"; issue: "10"; pages: 
            "1390-1395"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2009)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (04-FEB-2009) Contact:Takuo Onizuka Toray Research Center, Inc., 
            Biological Science Laboratories; 1111 Tebiro, Kamakura, Kanagawa 
            248-0036, Japan"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    401..406
                     /label=XbaI cloning site
                     /note="XbaI cloning site"
     misc_feature    407..412
                     /label=PstI cloning site
                     /note="PstI cloning site"
     misc_feature    413..418
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     misc_feature    417..422
                     /label=SmaI cloning site
                     /note="SmaI cloning site"
     misc_feature    421..426
                     /label=KpnI cloning site
                     /note="KpnI cloning site"
     misc_feature    427..432
                     /label=ClaI cloning site
                     /note="ClaI cloning site"
     misc_feature    433..438
                     /label=EciRI cloning site
                     /note="EciRI cloning site"
     CDS             complement(625..3456)
                     /codon_start=1
                     /gene="orf943"
                     /product="ORF943"
                     /function="essential for the replication of the plasmid in 
                     cyanobacteria"
                     /label=orf943
                     /protein_id="BAH22361.1"
                     /translation="MTPYEYLLYSDEPALRRNDGRLRDTWLKRYAFVEHGGWWCSGIDI
                     KTGKDSLWGCFKGDRPRKDREDKKPIKYEHPPRVATEIFTLKVDRGTWRKIAKRHKVEL
                     PETDQGFWEWVLAHPELPIIITEGAKKAGALLTAGYCAIGLPGIYNGYRTPKNDHGEPM
                     RQLRHLIPELDLLAKNNRAIAFCFDQDKKPKTIKAVNGAIQTTGALLEKAGAKVSVITW
                     HQDAKGVDDLIVEHGAKALHNRYKHRKPLAVWEMDNLTDITTQVDLTVDQRYLDIDPRA
                     IPKDAQIIFIKSAKGTGKTEWLGKIVKLAQDDCARVLVLTHRIQLAKELARRLDIDHIS
                     ELDSSPTGGALGMAMCIDSLHPDSQAHFNFMEWHGAHIVLDEIEQVLGHALGSSTCTQD
                     RAKILETFYNLILYALRTGGKLYCSDADLSPISYELIKYILDGCEFKPFTILNTYKPCL
                     EQQRDLFFYEGNDPRDLLTNLRQAIENGEKTLVFTAAQKTASTYSTQNLESLFREKYPD
                     KRILRIDAESVAEPGHPAYGCIDSLNAILPLYDIVLCSPAVETGVSIDIKDHFDSVWGM
                     GSGVQTVNGFCQGLERLRDNVPRHVWIPKFSPHSNRIANGGYTAKAIARDQHRYAELTH
                     KLIGEHAAECSGLEDSLKPFLWAYCRYAALANRGFGSYREAILNKLLSEGYVQKDLSEI
                     DPALAKDYRDELKAVKDHNYLQERVAISKVENPDDRQYEKLKRQRAKSETERHQERHGK
                     LSRSYGLTVTPELVEKDDDGWYSQLQLEYYLTVGKAFCSARDRAKYDQLQHEGFVFKPD
                     INRRSLSPKIHLLELLNIHQFLKPGVTFTGASLEGFKENCLRYAKPIKWILGRTITDKM
                     SPLEIAQALLGKLDRKLEYKGRFGSRDNRQRVYEAIAPNDQREKVFAHWLQRDQAKLGA
                     VSNPCINRFIQEA"
     gene            complement(625..3456)
                     /gene="orf943"
                     /label=orf943
     primer_bind     complement(3657..3673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3681..3697)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3705..3735)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3750..3771)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4059..4647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4821..5678)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5679..5783)
                     /label=AmpR promoter