pANY6 vector (V009491)

LOCUS       40924_4559        4094 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pANY6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu
            Y.
  TITLE     Three novel Escherichia coli vectors for convenient and efficient 
            molecular biological manipulations
  JOURNAL   J. Agric. Food Chem. (2018) In press
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu
            Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2018) College of Biosciences and Biotechnology, 
            Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, 
            Liaoning 110161, P. R. China.
REFERENCE   3  (bases 1 to 4094)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4094)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Agric. 
            Food Chem. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAY-2018) College of Biosciences and Biotechnology, Shenyang 
            Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 
            110161, P. R. China."
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      53..641
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        718..736
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             768..790
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    798..1784
                     /label=ELP tag
                     /note="ELP tag"
     misc_feature    1785..2294
                     /label=intein
                     /note="intein"
     regulatory      2295..2326
                     /label=multiple cloning sites (MCS)-1
                     /note="multiple cloning sites (MCS)-1"
                     /regulatory_class="other"
     CDS             2657..2959
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      2989..3026
                     /label=multiple cloning sites (MCS)-2
                     /note="multiple cloning sites (MCS)-2"
                     /regulatory_class="other"
     terminator      3054..3101
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(3169..3197)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(3213..4025)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"