pANY3 vector (V009492)

LOCUS       40924_4554        3734 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pANY3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu
            Y.
  TITLE     Three novel Escherichia coli vectors for convenient and efficient 
            molecular biological manipulations
  JOURNAL   J. Agric. Food Chem. (2018) In press
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu
            Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) College of Biosciences and Biotechnology, 
            Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, 
            Liaoning 110161, P. R. China.
REFERENCE   3  (bases 1 to 3734)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Agric. 
            Food Chem. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2018) College of Biosciences and Biotechnology, Shenyang 
            Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 
            110161, P. R. China."
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..18
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      80..127
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(195..223)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(239..1051)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1173..1761
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1845..2555)
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     regulatory      2603..2630
                     /label=pR promoter
                     /note="pR promoter"
                     /regulatory_class="promoter"
     RBS             2644..2652
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      2817..2845
                     /label=pL promoter
                     /note="pL promoter"
                     /regulatory_class="promoter"
     regulatory      2985..3024
                     /label=multiple cloning sites (MCS)-1
                     /note="multiple cloning sites (MCS)-1"
                     /regulatory_class="other"
     CDS             3354..3656
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      3689..3734
                     /label=multiple cloning sites (MCS)-2
                     /note="multiple cloning sites (MCS)-2"
                     /regulatory_class="other"