pAK-TAG-T vector (V009538)

LOCUS       V009538                 4115 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009538
VERSION     V009538
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Luo D, Wen C, Zhao R, Liu X, Liu X, Cui J, Liang JG, Liang P.
  TITLE     High Level Expression and Purification of Recombinant Proteins from
            Escherichia coli with AK-TAG
  JOURNAL   PLoS ONE 11 (5), E0156106 (2016)
   PUBMED   27214237
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Luo D.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4115)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4115)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "5"; pages: "E0156106"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-APR-2016) Department of Biochemistry "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             127..144
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             151..792
                     /gene="adk"
                     /label="Adenylate kinase"
                     /note="Adenylate kinase from Escherichia coli (strain K12 /
                     DH10B). Accession#: B1XFR1"
     CDS             799..816
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    817..846
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     terminator      862..956
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             1000..1656
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      1724..1810
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1965..2105
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2291..2879)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3053..3910)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3911..4015)
                     /label="AmpR promoter"