pAgc-A2-bgyg-3'N vector (V009570)

LOCUS       40924_4110       13100 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pAgc-A2-bgyg-3'N DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Ikeda Y, Ito K, Izumi Y, Shinomura T.
  TITLE     A candidate enhancer element responsible for high-level expression 
            of the aggrecan gene in chondrocytes
  JOURNAL   J. Biochem. 156 (1), 21-28 (2014)
  PUBMED    24554731
REFERENCE   2  (bases 1 to 13100)
  AUTHORS   Ikeda Y, Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and 
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan URL :http://www.tmd.ac.jp/
REFERENCE   3  (bases 1 to 13100)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13100)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2014"; volume: "156"; issue: "1"; pages: "21-28"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 
            113-8549, Japan URL :http://www.tmd.ac.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      17..550
                     /gene="bgyg"
                     /label=rat Agc1 promoter sequence
                     /note="rat Agc1 promoter sequence"
                     /regulatory_class="promoter"
     exon            551..931
                     /gene="bgyg"
                     /note="rat Agc1 exon 1 sequence"
     intron          932..4705
                     /gene="bgyg"
     misc_binding    1560..1964
                     /gene="bgyg"
                     /label=SOX9 binding site
                     /bound_moiety="SOX9"
                     /note="A2"
     CDS             4887..7928
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             7944..8978
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     promoter        complement(9969..9987)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10005..10021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10029..10045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10053..10083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10098..10119)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10407..10995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11169..12026)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12027..12131)
                     /label=AmpR promoter
     rep_origin      12465..12920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     13061..13077
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"