pCCRE vector (V008660)

LOCUS       40924_9276       11484 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCCRE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Ma BG, Ma C, Niu JX.
  TITLE     Gene expression controlled by inducible Cre/loxP recombination 
            system in transgenic tobacco
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11484)
  AUTHORS   Ma BG, Ma C, Niu JX.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2007) Department of Horticulture, Agriculture 
            College Shihezi University, Shihezi, Xinjiang 832000, P.R. China
REFERENCE   3  (bases 1 to 11484)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11484)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2007) Department of Horticulture, Agriculture College 
            Shihezi University, Shihezi, Xinjiang 832000, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        495..840
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     849..865
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             963..1991
                     /label=Cre
                     /note="site-specific recombinase"
     regulatory      2150..2402
                     /label=NOS
                     /note="NOS"
                     /regulatory_class="terminator"
     terminator      2150..2402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2411..2427)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2745..2997
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3019..3043
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4343..4969
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5406..6470
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6539..6733
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7077..7217
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7403..7991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8081..8872)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9297..9321
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9399..9573)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9616..10638)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(10706..11383)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"