pCB119-Edim6 vector (V008701)

LOCUS       V008701                 7878 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008701
VERSION     V008701
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Dennehy M, Bourn W, Steele D, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Dennehy M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2005) Virology, University of Cape Town, Anzio
            Road Observatory, Cape Town, WP 7925, South Africa
REFERENCE   3  (bases 1 to 7878)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-APR-2005) Virology, University of Cape Town, Anzio Road
            Observatory, Cape Town, WP 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      163..168
                     /label="derived from BCG hsp60 promoter region"
                     /note="derived from BCG hsp60 promoter region"
                     /regulatory_class="minus_35_signal"
     regulatory      186..191
                     /label="derived from BCG hsp60 promoter region"
                     /note="derived from BCG hsp60 promoter region"
                     /regulatory_class="minus_10_signal"
     misc_feature    198
                     /label="BCG hsp60 transcription start site"
                     /note="BCG hsp60 transcription start site"
     regulatory      364..370
                     /regulatory_class="ribosome_binding_site"
     CDS             382..1749
                     /codon_start=1
                     /product="hsp60-lysA chimera"
                     /label="hsp60-lysA chimera"
                     /protein_id="AAY46601.1"
                     /translation="MAKTIADPDVNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQ
                     EYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYAAKAFLCSEVARWISEEGLCLDVCTG
                     GELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMTEIERLDAIAGEAGI
                     VQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGLHSH
                     IGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPI
                     AELAAKLGTIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRR
                     YVSVDGGMSDNIRTGLYGAQYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPTI
                     FGPAIWLRLPPPALLLFAVESLQHGRPSRCGSGARGNARLVLRRETVDDLLSLEVR"
     CDS             1877..2689
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    3771..5666
                     /note="OriM; mycobacterial origin of replication region;
                     similar to pAL5000 in GenBank Accession Number M60875"
     CDS             5698..6330
                     /gene="tmk"
                     /label="Thymidylate kinase"
                     /note="Thymidylate kinase from Mycobacterium tuberculosis
                     (strain ATCC 25618 / H37Rv). Accession#: P9WKE1"
     CDS             6412..7749
                     /codon_start=1
                     /product="19 kDa-rotavirus EDIM VP6 chimera"
                     /label="19 kDa-rotavirus EDIM VP6 chimera"
                     /note="contains Mycobacterium tuberculosis 19 kDa secretory
                     signal sequence"
                     /protein_id="AAY46603.1"
                     /translation="MKRGLTVAVAGAAILVAGLSGCSSNKSTTGSGETTTAAGTTASPG
                     GGPMDVLYSISRTLKDARDKIVEGTLYSNVSDLIQQFNQMLVTMNGNEFQTGGIGNLPL
                     RNWNFDFGLLGTTLLNLDANYVESARTTIDYFVDFIDNVCMDEMMRESQRNGIAPQSDA
                     LRKLSGVKFRRINFNNSSEYIENWNLQNRRQRTGFTFHKPNIFPYSASFTLNRSQPQHD
                     NLMGTMWLNAGSEIQVAGFDYSCAINAPANIQQFEHIVQLRRVLTTATITLLPDAERFS
                     FPRVINSADGATTWYFNPVILRPNNVEVEFLLNGQVINTYQARFGTIVARNFDTIRLSF
                     QLMRPPNMTPAVTALFPNAQPFEHHATVGLTLRIDSAICESVLADASETMLANVTSVRQ
                     EYAIPVGPVFPPGMNWTDLITNYSPSREDNLQRVFTVASIRSMLVK"
     terminator      7796..7842
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"