pCAT-3' vector (V008711)

LOCUS       40924_9071        4454 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAT-3', complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Sarafanov AG, Kondratov RV, Zakhar'ev VM, Prasolov VS, Kozlov,Iu.V.
  TITLE     [Gene for the human tissue activator plasminogen: regulation of 
            expression by signals, located in the 3'-untranslated region]
  JOURNAL   Mol. Biol. (Mosk.) 33 (3), 413-422 (1999)
  PUBMED    10519114
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Sarafanov AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2002) Biochemistry, American Red Cross, Holland 
            Laboratory, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
REFERENCE   3  (bases 1 to 4454)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4454)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            (Mosk.)"; date: "1999"; volume: "33"; issue: "3"; pages: "413-422"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2002) Biochemistry, American Red Cross, Holland Laboratory, 
            15601 Crabbs Branch Way, Rockville, MD 20855, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     363..379
                     /label=pUC/M13 forward primer
                     /note="pUC/M13 forward primer"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        412..608
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             646..1302
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     intron          1471..1536
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1666..1686
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     primer_bind     1724..1740
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1768..1784)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1811..1945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1947..2183
                     /label=SV40 enhancer
                     /note="SV40 enhancer"
                     /regulatory_class="enhancer"
     primer_bind     complement(2235..2251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2259..2275)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2283..2313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2328..2349)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2637..3223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3397..4254)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4255..4359)
                     /label=AmpR promoter