pCAMBIA1300-VE2-VE3 vector (V008734)

LOCUS       V008734                13725 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008734
VERSION     V008734
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13725)
  AUTHORS   Shi XG, Zhuang WJ.
  TITLE     Binary vector pCAMBIA1300AT for making homogentisate
            phytyltransferase and MPBQ/MSBQ methyltransferase over expression in
            seed
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13725)
  AUTHORS   Zhuang WJ, Shi XG.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2008) Crop Science Department, Oil Crop
            Institution, Fujian Agriculture, Jinshan, Fuzhou, Fujian 350002,
            P.R. China
REFERENCE   3  (bases 1 to 13725)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13725)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-OCT-2008) Crop Science Department, Oil Crop Institution, Fujian
            Agriculture, Jinshan, Fuzhou, Fujian 350002, P.R. China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      8..260
                     /label="NOS"
                     /note="NOS"
                     /regulatory_class="terminator"
     terminator      8..260
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(284..1462)
                     /gene="HPT1"
                     /label="Homogentisate phytyltransferase 1, chloroplastic"
                     /note="Homogentisate phytyltransferase 1, chloroplastic
                     from Arabidopsis thaliana. Accession#: Q8VWJ1"
     regulatory      1474..2350
                     /label="soybean 7S promoter"
                     /note="soybean 7S promoter"
                     /regulatory_class="promoter"
     terminator      complement(2363..2615)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2636..3649)
                     /gene="VTE3"
                     /label="2-methyl-6-phytyl-1,4-hydroquinone
                     methyltransferase, chloroplastic"
                     /note="2-methyl-6-phytyl-1,4-hydroquinone
                     methyltransferase, chloroplastic from Arabidopsis thaliana.
                     Accession#: Q9LY74"
     regulatory      3661..4805
                     /label="Brassica napus 2S seed storage protein promoter"
                     /note="Brassica napus 2S seed storage protein promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(4827..4843)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5046..5070
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6370..6996
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7433..8497
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8566..8760
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9104..9244
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9430..10018)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10108..10899)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    11324..11348
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(11426..11600)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11643..12665)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(12733..13410)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    13601..13622
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        13637..13667
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    13675..13691
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13699..13715
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"