pCAMBIA1300-VE2-7S vector (V008735)

LOCUS       V008735                11288 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008735
VERSION     V008735
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Shi XG, Zhuang WJ.
  TITLE     Binary vector pCAMBIA1300-VE2-7S for making homogentisate
            phytyltransferase over expression in seed
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Zhuang WJ, Shi XG.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2008) Crop Science Department, Oil Crop
            Institution, Fujian Agriculture, Jinshan, Fuzhou, Fujian 350002,
            P.R. China
REFERENCE   3  (bases 1 to 11288)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11288)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-OCT-2008) Crop Science Department, Oil Crop Institution, Fujian
            Agriculture, Jinshan, Fuzhou, Fujian 350002, P.R. China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(8..260)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(284..1462)
                     /gene="HPT1"
                     /label="Homogentisate phytyltransferase 1, chloroplastic"
                     /note="Homogentisate phytyltransferase 1, chloroplastic
                     from Arabidopsis thaliana. Accession#: Q8VWJ1"
     regulatory      1474..2350
                     /label="soybean 7S promoter"
                     /note="soybean 7S promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2390..2406)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2609..2633
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3933..4559
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4996..6060
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6129..6323
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6667..6807
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6993..7581)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7671..8462)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    8887..8911
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8989..9163)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9206..10228)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(10296..10973)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11164..11185
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11200..11230
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11238..11254
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11262..11278
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"