pCAMBIA1300-TMT-2S vector (V008736)

LOCUS       V008736                11375 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008736
VERSION     V008736
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Shi XG, Zhuang WJ.
  TITLE     Binary vector pCAMBIA1300-TMT-2S for making gamma-tocopherol
            methyltransferase over expression in seed
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   Zhuang WJ, Shi XG.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2008) Crop Science Department, Oil Crop
            Institution, Fujian Agriculture, Jinshan, Fuzhou, Fujian 350002,
            P.R. China
REFERENCE   3  (bases 1 to 11375)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11375)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-OCT-2008) Crop Science Department, Oil Crop Institution, Fujian
            Agriculture, Jinshan, Fuzhou, Fujian 350002, P.R. China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(8..260)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(280..1323)
                     /gene="VTE4"
                     /label="Tocopherol O-methyltransferase, chloroplastic"
                     /note="Tocopherol O-methyltransferase, chloroplastic from
                     Arabidopsis thaliana. Accession#: Q9ZSK1"
     regulatory      1341..2501
                     /label="Brassica napus 2S seed storage protein promoter"
                     /note="Brassica napus 2S seed storage protein promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2508..2524)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2727..2751
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4051..4677
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5114..6178
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6247..6441
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6785..6925
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7111..7699)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7789..8580)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9005..9029
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9107..9281)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9324..10346)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(10414..11091)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11282..11303
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11318..11348
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11356..11372
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."