Basic Vector Information
- Vector Name:
- pLenti-puro-ARID1A
- Antibiotic Resistance:
- Ampicillin
- Length:
- 13943 bp
- Type:
- Mammalian Expression, Lentiviral
- Replication origin:
- ori
- Selection Marker:
- Puromycin
- Copy Number:
- High Copy
- Promoter:
- RSV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- CMV-fwd
- 3' Primer:
- BGH-rev
pLenti-puro-ARID1A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLenti-puro-ARID1A vector Sequence
LOCUS pLenti-puro-ARID 13943 bp DNA circular SYN 13-MAY-2021
DEFINITION Tetracycline-inducible lentiviral expression of human ARID1A; 3rd
generation lentiviral vector.
ACCESSION .
VERSION .
KEYWORDS pLenti-puro-ARID1A.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13943)
AUTHORS Guan B, Wang TL, Shih IeM
TITLE ARID1A, a factor that promotes formation of SWI/SNF-mediated
chromatin remodeling, is a tumor suppressor in gynecologic cancers.
JOURNAL Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.
PUBMED 21900401
REFERENCE 2 (bases 1 to 13943)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 13943)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cancer
Res."; date: "2011-11-1"; volume: "71(21)"; pages: "6718-27. Epub
2011 Sep 7"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..13943
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 107..128
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 242..260
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 288..514
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 515..695
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 742..867
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1360..1593
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1778..1822
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1971..2012
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
enhancer 2101..2403
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2404..2607
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 2609..2627
/label=tet operator
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2630..2648
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 2732..9586
/gene="ARID1A"
/label=ARID1A
/note="AT-rich interactive domain-containing protein 1A
from Homo sapiens. Accession#: O14497"
CDS 9614..9655
/label=V5 tag
/note="epitope tag from simian virus 5"
CDS 9665..9682
/label=6xHis
/note="6xHis affinity tag"
primer_bind complement(9705..9722)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
primer_bind complement(9734..9754)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9739..10068
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9919..10054
/label=SV40 ori
/note="SV40 origin of replication"
CDS 10078..10674
/label=PuroR
/note="puromycin N-acetyltransferase"
LTR 10749..10982
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 11054..11188
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 11215..11350
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(11371..11389)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(11399..11416)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(11399..11415)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11408..11430)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 11557..12012
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 12038..12142
/label=AmpR promoter
CDS 12143..13000
/label=AmpR
/note="beta-lactamase"
rep_origin 13174..13762
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 13916..13933
/label=L4440
/note="L4440 vector, forward primer"
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