pBSK-Gus vector (V009030)

LOCUS       40924_7441        3827 bp DNA     circular SYN 17-DEC-2018
DEFINITION  RNA interference vector pBSK-Gus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Narwade AV, Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG.
  TITLE     Hairpin RNA generating vector designed by cloning 878 bp Gus 
            fragment from E. coli GUSA (N358Q) amplified from pCAMBIA-1302 as 
            stuffer. The vector can be utilized for generating dsRNA of a given 
            sequence by cloning the targeted DNA in sense and antisense 
            orientations flanking the stuffer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Narwade AV, Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG.
  TITLE     RNA interference vector pBSK-Gus, to generate inverted repeat of a 
            given sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3827)
  AUTHORS   Chakrabarty PK, Narwade AV, Kalbande BB, Chavhan RL, Warade JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2010) Division of Crop Improvement, Central 
            Institute for Cotton Research, Post Bag No. 02, Shankar Nagar P.O., 
            Nagpur, Maharashtra 440010, India
REFERENCE   4  (bases 1 to 3827)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3827)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2010) Division of Crop Improvement, Central Institute for 
            Cotton Research, Post Bag No. 02, Shankar Nagar P.O., Nagpur, 
            Maharashtra 440010, India"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    701..706
                     /label=EcoRI site
                     /note="EcoRI site"
     gene            707..1584
                     /gene="gusA"
                     /label=gusA
                     /note="beta-glucuronidase; stuffer fragment for RNA 
                     interference; derived from Escherichia coli vector 
                     pCAMBIA-1302; M358Q GusA fragment"
     primer_bind     complement(1586..1602)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1639..1657)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1678..1694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1702..1718)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1726..1756)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1771..1792)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2080..2668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2842..3699)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3700..3804)
                     /label=AmpR promoter