pBSc243C vector (V009050)

LOCUS       V009050                 4969 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009050
VERSION     V009050
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
   PUBMED   29074996
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden,
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep";
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher
            Weg 20b, Dresden 01627, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    249..305
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      604..698
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             877..1689
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            1859..1967
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      2061..2649
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2706..3536)
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3550..3901
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             complement(4069..4716)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     terminator      complement(4877..4963)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"