pBS437V vector (V009065)

LOCUS       V009065                 6399 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009065
VERSION     V009065
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Tooley AJ, Glazer AN.
  TITLE     Biosynthesis of the cyanobacterial light-harvesting polypeptide
            phycoerythrocyanin holo-alpha subunit in a heterologous host
  JOURNAL   J. Bacteriol. 184 (17), 4666-4671 (2002)
   PUBMED   12169589
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Tooley AJ, Glazer AN.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2003) Molecular and Cell Biology, University of
            California, Berkeley, 142 LSA no. 3200, Berkeley, CA 94720-3200, USA
REFERENCE   3  (bases 1 to 6399)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6399)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2002"; volume: "184"; issue: "17"; pages:
            "4666-4671"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2003) Molecular and Cell Biology, University of California,
            Berkeley, 142 LSA no. 3200, Berkeley, CA 94720-3200, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            10..195
                     /gene="rop"
                     /label="rop"
                     /note="coding region not determined; ROP'; repressor of
                     primer protein variant; regulation of plasmid replication;
                     helps maintain medium plasmid copy number"
     misc_feature    297..439
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(625..1213)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1497..2285
                     /codon_start=1
                     /gene="aadA"
                     /product="AadA"
                     /label="aadA"
                     /note="confers resistance to streptomycin and to
                     spectinomycin via inactivation of the drugs by specific
                     adenylylation"
                     /protein_id="AAP45701.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1497..2285
                     /gene="aadA"
                     /label="aadA"
     CDS             2506..3585
                     /label="lacI"
                     /note="lac repressor"
     regulatory      3820..3825
                     /label="trc promoter"
                     /note="trc promoter"
                     /regulatory_class="minus_35_signal"
     regulatory      3843..3849
                     /label="trc promoter"
                     /note="trc promoter"
                     /regulatory_class="minus_10_signal"
     misc_feature    3855..3856
                     /label="transcription initiation site from trc promoter"
                     /note="transcription initiation site from trc promoter"
     protein_bind    3857..3873
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      3882..3887
                     /gene="pecA"
                     /regulatory_class="ribosome_binding_site"
     CDS             3900..3917
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3939..3959
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             3966..4451
                     /gene="pecA"
                     /label="Phycoerythrocyanin alpha chain"
                     /note="Phycoerythrocyanin alpha chain from Nostoc sp.
                     (strain PCC 7120 / SAG 25.82 / UTEX 2576). Accession#:
                     P35796"
     gene            4487..5259
                     /gene="pecE"
                     /label="pecE"
     regulatory      4487..4492
                     /gene="pecE"
                     /regulatory_class="ribosome_binding_site"
     CDS             4498..5259
                     /codon_start=1
                     /gene="pecE"
                     /product="PecE"
                     /label="pecE"
                     /note="catalyzes both the covalent attachment of
                     phycocyanobilin to the phycoerythrocyanin alpha subunit and
                     its concurrent isomerization to phycobiliviolin"
                     /protein_id="AAP45704.1"
                     /translation="MTAEPILSPETAIAALSGEDNQIRYYAAWWLGKHNVQAGFTALCV
                     ALFDERYRIPSGGYPLRRQAARALGLLKNPQAVPALIAALECDEDLRLREAVICSLAAI
                     GDKRAVTPLLNLLQSSQAQPYEALIEALATLQVWSARPQIEPFLQHYSERVQCAAARYM
                     YLLTQESEYIERIVKNLNHDNMYLRWAAVFDLGAVAHQQAVQAILTAQVPNSLKLLNLK
                     RILEAMLNNNSVNHEKAALLFGAIDDLLIQL"
     gene            5268..5800
                     /gene="pecF"
                     /label="pecF"
     regulatory      5268..5273
                     /gene="pecF"
                     /regulatory_class="ribosome_binding_site"
     CDS             5279..5800
                     /codon_start=1
                     /gene="pecF"
                     /product="PecF"
                     /label="pecF"
                     /note="catalyzes both the covalent attachment of
                     phycocyanobilin to the phycoerythrocyanin alpha subunit and
                     its concurrent isomerization to phycobiliviolin"
                     /protein_id="AAP45705.1"
                     /translation="MNQASLSVDAITNLIEAFHHHHPAVRSAAVDELIKLGSITVNLLI
                     AAYDDSQDQGFQAQIIQVLAQIGDAKALKLLAEVVGTSVANHCQGNVRRIAARGLGKIA
                     STTSNTEIINNAQEKLIWALLTPEDWGLRYAAAVSLQEIATPKAKAALQQAIAQETDPV
                     VRSRMAIALS"
     terminator      6136..6222
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6314..6341
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"