pBS414V vector (V009070)

LOCUS       V009070                 6587 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009070
VERSION     V009070
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Tooley AJ, Cai YA, Glazer AN.
  TITLE     Biosynthesis of a fluorescent cyanobacterial C-phycocyanin
            holo-alpha subunit in a heterologous host
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 98 (19), 10560-10565 (2001)
   PUBMED   11553806
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Tooley AJ, Cai YA, Glazer AN.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2003) Molecular and Cell Biology, University of
            California, Berkeley, 142 LSA no. 3200, Berkeley, CA 94720-3200, USA
REFERENCE   3  (bases 1 to 6587)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6587)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2001"; volume: "98"; issue: "19"; pages:
            "10560-10565"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2003) Molecular and Cell Biology, University of California,
            Berkeley, 142 LSA no. 3200, Berkeley, CA 94720-3200, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            10..195
                     /gene="rop"
                     /label="rop"
                     /note="coding region not determined; repressor of primer
                     protein variant; regulation of plasmid replication;
                     maintains medium plasmid copy number"
     misc_feature    297..439
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(625..1213)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1497..2285
                     /codon_start=1
                     /gene="aadA"
                     /product="AadA"
                     /label="aadA"
                     /note="confers resistance to streptomycin and to
                     spectinomycin via inactivation of the drugs by specific
                     adenylylation"
                     /protein_id="AAP43512.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1497..2285
                     /gene="aadA"
                     /label="aadA"
     CDS             2506..3585
                     /label="lacI"
                     /note="lac repressor"
     regulatory      3820..3825
                     /label="trc promoter"
                     /note="trc promoter"
                     /regulatory_class="minus_35_signal"
     regulatory      3843..3849
                     /label="trc promoter"
                     /note="trc promoter"
                     /regulatory_class="minus_10_signal"
     misc_feature    3855..3856
                     /note="transcription initiation site from the trc promoter"
     protein_bind    3857..3873
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      3882..3887
                     /gene="cpcA"
                     /regulatory_class="ribosome_binding_site"
     CDS             3900..3917
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3939..3959
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             3966..4451
                     /gene="cpcA"
                     /label="C-phycocyanin alpha subunit"
                     /note="C-phycocyanin alpha subunit from Synechocystis sp.
                     (strain PCC 6803 / Kazusa). Accession#: Q54715"
     gene            4483..5313
                     /gene="cpcE"
                     /label="cpcE"
     regulatory      4483..4488
                     /gene="cpcE"
                     /regulatory_class="ribosome_binding_site"
     CDS             4495..5313
                     /codon_start=1
                     /gene="cpcE"
                     /product="CpcE"
                     /label="cpcE"
                     /note="required for the covalent attachment of
                     phycocyanobilin to the alpha subunit apoprotein of
                     C-phycocyanin"
                     /protein_id="AAP43515.1"
                     /translation="MSEPNLNPAYTLDQAIANLQQTEDASARYYAAWWIGRFRAAQPET
                     IAALLVALEDETDRSPDGGYPLRRNAAKALGKLGDRQVVPALIKALECEDYYVRESAAQ
                     ALEGLGDARAMAPLMAKLTGGLAAAQLVEGKPHLAQPYEAIIEALGTLQAVESIGLIEP
                     FLEHFSPKVQYAAARALFQLTGDNRYGDLLITALGGTDLQLRRSAMMDLGATGYLPGAQ
                     AIAKAFAENSLKLIALRDLWATHRQRQASSESKALSPASRQILELMDSLL"
     gene            5330..5988
                     /gene="cpcF"
                     /label="cpcF"
     regulatory      5330..5335
                     /gene="cpcF"
                     /regulatory_class="ribosome_binding_site"
     CDS             5344..5988
                     /codon_start=1
                     /gene="cpcF"
                     /product="CpcF"
                     /label="cpcF"
                     /note="required for the covalent attachment of
                     phycocyanobilin to the alpha subunit apoprotein of
                     C-phycocyanin"
                     /protein_id="AAP43516.1"
                     /translation="MEGNSVVTPEIERLIQAVETADSAAKLVGAVRALAATRSPLAVPQ
                     LTTVLRYNNPGAAVAAVDGLIQIGDAAMTHLLANMDGYNYGARAWATRACAGIGDPRAL
                     ALLQEAALTDFALSVRRAAAKGLGFLRWQSLPQEEQETVQKAIYDTLIQVCEDPEWVVR
                     YGAIAGLENLAKQAQHYRQPLKDFLQSFVEQEPEAIVGERILWTLENIGPI"
     terminator      6324..6410
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6502..6529
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"