pBS-C5 vector (V009082)

LOCUS       40924_7146        3117 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning/transgenesis vector pBS-C5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Le T, Yu M, Williams B, Goel S, Paul SM, Beitel GJ.
  TITLE     CaSpeR5, a family of Drosophila transgenesis and shuttle vectors 
            with improved multiple cloning sites
  JOURNAL   BioTechniques 42 (2), 164 (2007)
  PUBMED    17373479
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Beitel GJ, Le T, Yu M, Williams B, Goel S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2006) BMBCB, Northwestern University, 2205 Tech 
            Dr., Evanston, IL 60091, USA
REFERENCE   3  (bases 1 to 3117)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2007"; volume: "42"; issue: "2"; pages: 
            "164"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2006) BMBCB, Northwestern University, 2205 Tech Dr., 
            Evanston, IL 60091, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..460
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     601..617
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        627..645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    661..906
                     /label=ppCasper5 multiple cloning site
                     /note="ppCasper5 multiple cloning site"
     promoter        complement(929..947)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(968..984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(992..1008)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1016..1046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1061..1082)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1370..1958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2132..2989)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2990..3094)
                     /label=AmpR promoter